Team:Paris Saclay/Notebook/July/12

From 2013.igem.org

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==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
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===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''====
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===='''Objective : obtaining BBa_K1155003, BBa_K1155007'''====
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===='''1 -Transformation of Bba_I732019, Bba_B0015, Bba_B0017, Bba_B0010'''====
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===='''1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017'''====
Zhou
Zhou
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Transformation of 07/10/13 of Bba_I732019 didn't works. We will do it again. We obtain two colonies of 07/10/13 transformation of Bba_B0010. We will extract Bba_B0010 and do the transformation again.
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Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.  
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We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.
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===='''3 -Extraction of Bba_B0010 from DH5α'''====
 
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Sheng
 
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Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
 
==='''B - PCB sensor system'''===
==='''B - PCB sensor system'''===
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===='''Objective : obtaining Bba_K1155001, Bba_K1155002 and BphR2'''====
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===='''Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2'''====
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===='''1 - Stock of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2'''====
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===='''1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2'''====
Zhou
Zhou
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* Culture : 1µL
* Culture : 1µL
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===='''2 - Extraction of clones 5, 6, 7, 8 for BphA1, clones 5, 6 for BphR1 and clones 3, 4 for BphR2 from DH5α'''====
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===='''2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α'''====
Abdou, Sheng
Abdou, Sheng
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Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
Protocol : [[Team:Paris_Saclay/extraction|High copy plamid extraction]]
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===='''3 - Streak of BphA1 and BphR2'''====
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===='''3 - Streak of BBa_K1155002 and BphR2'''====
Anaïs
Anaïs
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We strek 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.  
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We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.  
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Latest revision as of 09:55, 30 September 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : July 12

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155003, BBa_K1155007

1 -Transformation of BBa_I732019, BBa_B0015, BBa_B0017

Zhou

Transformation of 07/10/13 of BBa_I732019 didn't works. We will do it again.

Protocol : Bacterial transformation

2 -Design of RBS-AmilCP oligos

Abdou, Anaïs

We used software gene manager to find the correct oligopeptides for amplification of RBS-Amil CP.


B - PCB sensor system

Objective : obtaining BBa_K1155001, BBa_K1155002 and BphR2

1 - Stock of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2

Zhou

Used quantities :

  • Glycerol 85% : 425
  • Culture : 1µL

2 - Extraction of clones 5, 6, 7, 8 for BBa_K1155002, clones 5, 6 for BBa_K1155001 and clones 3, 4 for BphR2 from DH5α

Abdou, Sheng

Protocol : High copy plamid extraction

3 - Streak of BBa_K1155002 and BphR2

Anaïs

We streak 25 colonies of 07/10/13 transformation of BphA1 and BphR2 in new solid culture.


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