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Team:TU-Delft/FinalApplication
From 2013.igem.org
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Revision as of 12:58, 30 September 2013
BandAid Application
Experiments
Aim: To check if it is feasible for the peptides to pass through the selected membranes (Cellulose Nitrate Membrane and PTFE Membrane).
Procedure:- Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.
- The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be 0.49mM for signiferin and 0.34mM for maximim
- In order to make diffusion measurable, we folded the membrane like a filter paper and placed it in the peptide solution
- The membrane is add
| Absorbance(Maximim) | Absorbance(Signiferin) | Concentration in mM Maximim | Concentration in mM Signiferin | |
| ∅ | 2.83 | 1.76 | 1.89 | 1.18 |
| t0 | 0 | 0 | 0 | 0 |
| t10 | 0.4 | 0.64 | 0.26 | 0.42 |
| t20 | 0.93 | 0.71 | 0.62 | 0.47 |
| t30 | 1.98 | 1.46 | 1.32 | 0.97 |
