Chemical tools

There are 6 enzyms involved in the natural process of the enterobactin production:

• EntA :
• EntB :
• EntC :
• EntD :
• EntE :
• EntF :

The production of each one of those enzymes can thus become a rate-limiting-step, when it comes to mass enterobactin production.

For now, we consider each one of these steps as a simple chemical reaction:

We are using the enzymatic kinetic model of Michaelis-Menten, which divides each reaction into two consecutives steps:

The speed of the reaction is calculated as below:

The steady state approximation gives us:

And thus,

where and are classic kinetic parameters.

Conclusion:

For simple enzymatic reactions (one reagent, one product and one enzyme) with the steady state approximation, we can directly determine the speed:

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