Team:Evry/ChemicalTools

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where <img src="https://static.igem.org/mediawiki/2013/6/68/Km.jpg"/> and <img src="https://static.igem.org/mediawiki/2013/6/69/Kcat.jpg"/> are classic kinetic parameters.
where <img src="https://static.igem.org/mediawiki/2013/6/68/Km.jpg"/> and <img src="https://static.igem.org/mediawiki/2013/6/69/Kcat.jpg"/> are classic kinetic parameters.
</p>
</p>
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<h2>Conclusion:</h2>
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<h2>Important result:</h2>
<p>
<p>
For simple enzymatic reactions (<b>one</b> reagent, <b>one</b> product and <b>one</b> enzyme) with the steady state approximation, we can directly determine the speed:<br/><img src="https://static.igem.org/mediawiki/2013/0/0e/Resultat_final_vit_reac.jpg"/></p>
For simple enzymatic reactions (<b>one</b> reagent, <b>one</b> product and <b>one</b> enzyme) with the steady state approximation, we can directly determine the speed:<br/><img src="https://static.igem.org/mediawiki/2013/0/0e/Resultat_final_vit_reac.jpg"/></p>

Revision as of 09:24, 2 October 2013

Iron coli project

Chemical tools

There are 6 enzyms involved in the natural process of the enterobactin production:

  • EntA :
  • EntB :
  • EntC :
  • EntD :
  • EntE :
  • EntF :
The production of each one of those enzymes can thus become a rate-limiting-step, when it comes to mass enterobactin production.


For now, we consider each one of these steps as a simple chemical reaction:

We are using the enzymatic kinetic model of Michaelis-Menten, which divides each reaction into two consecutives steps:

The speed of the reaction is calculated as below:

The steady state approximation gives us:

And thus,

where and are classic kinetic parameters.

Important result:

For simple enzymatic reactions (one reagent, one product and one enzyme) with the steady state approximation, we can directly determine the speed:

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