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Team:Paris Saclay/Notebook/August/20
From 2013.igem.org
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(→1 - Electrophoresis of PCR products : BphR2 Part I) |
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*Well 1 : 6µL DNA Ladder | *Well 1 : 6µL DNA Ladder | ||
| - | *Well 2 : 40µL BphR2 | + | *Well 2 : 40µL BphR2 Part I+8µl of 6X loading dye |
*Gel : 0.8% | *Gel : 0.8% | ||
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| + | Expected sizes : | ||
| + | * BphR2 Part I : 178bp | ||
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We obtain a frangment at the right size. We can purify it. | We obtain a frangment at the right size. We can purify it. | ||
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====2 - Gel purification of PCR products : BphR2 Part I ==== | ====2 - Gel purification of PCR products : BphR2 Part I ==== | ||
Revision as of 14:33, 2 October 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Sequences analysis
Damir, XiaoJing
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Sequencies were good. We obtain : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Electrophoresis of PCR products : BphR2 Part I
Nadia
| [[]] |
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Expected sizes :
- BphR2 Part I : 178bp
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We obtain a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
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We lost our fragment. We will do the PCR again. |
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