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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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<h3>General protocols and <i>E. coli</i> protocols</h3> | <h3>General protocols and <i>E. coli</i> protocols</h3> | ||
<ul> | <ul> | ||
| - | <li><a href="/Team:Tuebingen/Notebook/Protocols/sob">SOB medium</a></li> | + | <li><a href="/Team:Tuebingen/Notebook/Protocols/sob-medium">SOB medium</a></li> |
<li><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/lb">LB medium</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/tae">TAE buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/tae">TAE buffer</a></li> | ||
Revision as of 21:22, 3 October 2013
Protocols
General protocols and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- Gelelectrophoresis
- Chemo-competent E. coli cells
- E. coli transformation
- pGEM Ligaton - ligation for TA-cloning of PCR products
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini-Prep
- Gelextraction
- Colony-PCR
Saccharomyces cerevisiae specific protocols
