Team:TU-Eindhoven/Notebook
From 2013.igem.org
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In this section you can find a small description of all the work we have done so far organized by the category it belongs to. | In this section you can find a small description of all the work we have done so far organized by the category it belongs to. | ||
- | {{:Team:TU-Eindhoven/Template:WeekList}}{{:Team:TU-Eindhoven/Template:Week | unit=Feb-Apr | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=May | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=June | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=July | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=August | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=September | nr=}}{{:Team:TU-Eindhoven/Template:WeekListEnd}} | + | {{:Team:TU-Eindhoven/Template:WeekList}}{{:Team:TU-Eindhoven/Template:Week | unit=Feb-Apr | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=May | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=June | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=July | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=August | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=September | nr=}}{{:Team:TU-Eindhoven/Template:Week | unit=October | nr=}}{{:Team:TU-Eindhoven/Template:WeekListEnd}} |
{{:Team:TU-Eindhoven/Template:WeekStart}} | {{:Team:TU-Eindhoven/Template:WeekStart}} | ||
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{{:Team:TU-Eindhoven/Template:TimelineEnd}} | {{:Team:TU-Eindhoven/Template:TimelineEnd}} | ||
- | {{:Team:TU-Eindhoven/Template:Timeline | day=23- | + | {{:Team:TU-Eindhoven/Template:Timeline | day=23-30 | month=Sept | year= }} |
''General Activities'' | ''General Activities'' | ||
* Regular team meetings | * Regular team meetings | ||
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''Human Practices'' | ''Human Practices'' | ||
* Editing the newly arrived revised "facts" | * Editing the newly arrived revised "facts" | ||
+ | |||
+ | {{:Team:TU-Eindhoven/Template:TimelineEnd}} | ||
+ | {{:Team:TU-Eindhoven/Template:TimelineBottom}} | ||
+ | {{:Team:TU-Eindhoven/Template:WeekEnd}} | ||
+ | |||
+ | {{:Team:TU-Eindhoven/Template:TimelineEnd}} | ||
+ | {{:Team:TU-Eindhoven/Template:Timeline | day=1-4 | month=Sept-Oct | year= }} | ||
+ | ''General Activities'' | ||
+ | * Regular team meetings | ||
+ | * Contacting the media for outreach purposes | ||
+ | * Adding the final touches to the poster and the presentation | ||
+ | * Adding final details to the wiki | ||
+ | * Adding last details on the judging form | ||
+ | |||
+ | |||
+ | ''Wetlab'' | ||
+ | * Protein purification | ||
+ | * SDS analysis | ||
+ | * Fluorescence analysis | ||
+ | * Filling in the characterization forms from our BioBricks on the registry | ||
+ | |||
+ | |||
+ | ''Drylab'' | ||
+ | * Double checking modeling content on the wiki | ||
+ | |||
+ | |||
+ | ''Human Practices'' | ||
+ | * Editing the newly arrived revised "facts" | ||
+ | * Making sure all the goodies for the Jamboree are good to go | ||
{{:Team:TU-Eindhoven/Template:TimelineEnd}} | {{:Team:TU-Eindhoven/Template:TimelineEnd}} |
Revision as of 21:57, 3 October 2013
![](https://static.igem.org/mediawiki/2013/6/6d/TU-Eindhoven_Images_LogoTransp.png)
![](https://static.igem.org/mediawiki/2013/3/32/TU-Eindhoven_Images_MRiGEM.png)
![](http://2013.igem.org/wiki/images/4/45/TU-Eindhoven_iGEM_small.png)
In this section you can find a small description of all the work we have done so far organized by the category it belongs to.
Feb-Apr
- Background research
- Selection of topic
- Further research
- Selection of project
- Further research
May
- Regular team meeting
- Looking for sponsors
- Month planning
Wetlab
- Researching about the promoters
- Researching about the possible proteins for CEST imaging
Drylab
- Defining what needs to be modeled and what type of model is the most appropriate for it
- Regular team meeting
- Looking for sponsors
Wetlab
- Researching and defining the killing mechanism
Drylab
- Starting the Molecular Dynamics model for the proteins selection.
- Regular team meeting
- Looking for sponsors
Wetlab
- Defining the needed materials
- Defining the needed equipment
Drylab
- Continuing the Molecular Dynamics model for the proteins selection.
- Regular team meeting
- Looking for sponsors
Wetlab
- Locating, booking and learning how to use the necessary equipment
Drylab
- Analyzing the results from the Molecular Dynamics model for the proteins selection and identifying the most useful proteins for CEST imaging
- Researching and defining the needed parameters for the analysis of the decoy sites through a stochastic simulations model
Human Practices
- Selecting a main project
- Regular team meeting
- Looking for sponsors
Wetlab
- Locating, booking and learning how to use the necessary equipment
Drylab
- Starting the stochastic simulations for the analysis of the decoy sites
Human Practices
- Selecting a main project
June
- Regular team meeting
- Looking for sponsors
- Collaborating with Purdue iGEM team
- Planning month activities
Wetlab
- General construct design
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
Human Practices
- Defining the structure of the fact checker project
- Regular team meeting
- Looking for sponsors
Wetlab
- Protein design
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
Human Practices
- Redacting the preliminary description and survey for collaborators of the fact checker project
- Regular team meeting
- Looking for sponsors
Wetlab
- Final design of the constructs
Drylab
- Continuing the stochastic simulations for the analysis of the decoy sites
Human Practices
- Revising the preliminary description and survey for collaborators of the fact checker project
- Regular team meeting
- Looking for sponsors
Wetlab
- Ordering the constructs
- Working on the protocols
Drylab
- Analyzing the results from the stochastic simulations for the analysis of the decoy sites
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
July
- Regular team meeting
- Looking for sponsors
- Planning month activities
Wetlab
- Finalizing the protocols
- Planning lab schedule
Drylab
- Starting the ODE model for the analysis of the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
- Regular team meeting
- Looking for sponsors
- Collaborating with Purdue iGEM team
Drylab
- Continuing the ODE model for the analysis of the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
- Regular team meetings
- Looking for sponsors
- Collaborating with Purdue iGEM team
Wetlab
- Creating agar plates
- Transforming DNA
- Plating bacteria
- Making LB medium
- Creating small cultures
- DNA retention from cultures
- Nano-drop tests
Drylab
- Analyzing the results from the ODE model for the analysis of the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
- Sending the survey to collaborators
- Regular team meetings
- Looking for sponsors
Wetlab
- Creating agar plates
- Transforming DNA
- Plating bacteria
- Making LB medium
- Creating small cultures
- DNA retention from cultures
- Nano-drop tests
Drylab
- Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
August
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
Wetlab
- Creating the Cultures
- Retaining the DNA
- Nanodrop Test
Drylab
- Merging the stochastic simulations model for the decoy sites with the ODE model for the FNR promoter
- Working on the pharmacokynetics model
Human Practices
- Researching for facts and myths related to Synthetic Biology to be checked
- Working on the fact checker web platform
- Redacting the first facts to be added on our fact checker platform
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
Wetlab
- Creating the cultures
- Retaining the DNA
- Nanodrop test
Drylab
- Improving the merged model
- Making an ODE model of the decoy sites
- Working on the pharmacokynetics model
Human Practices
- Working on the fact checker web platform
- Preparing the instructions and example files for collaborating teams
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
- Sharing our project with colleagues and faculty members
Wetlab
- Digestion
- Construct digestion
- Vector digestion
- PCR purification for the constructs
- PCR puricfication for the vectors
- Nanodrop results
- Continuing with the purification
- Vector purification
- Construct purification
- Ligation
- Preparing agar plates
- Transformation
- Plating
- Colony picking and PCR
- Culturing the ligation products
Drylab
- Sensitivity analysis of the merged model
- Estimating promotor rates
- Working on the pharmacokynetics model
Human Practices
- Working on the fact checker web platform
- Regular team meetings
- Looking for sponsors
- Working on outreach ideas
Wetlab
- Colony picking and PCR
- Small cultures and preparation for protein expression
- Miniprepping new constructs
- Sequencing
- Trnasformation and plating
- Expressing samples
- Protein purification
- Determining protein concentration
- SDS analysis
- Performing the protein expression in an anaerobic environment
- MRI experiments
Drylab
- Working on the pharmacokynetics model
Human Practices
- Fact checker platform is up and running
- Regular team meetings
- Looking for sponsors
- Contacting the media for outreach purposes
- Editing and sending our Brick of knowledge to the Copenhagen iGEM team for our collaboration with them.
Wetlab
- Expression cultures
- Anaerobic expression
- Protein expression
- Making the BioBricks
- SDS analysis
- BugBuster protocol
- SDS PAGE gel
Drylab
- Working on the pharmacokynetics model
Human Practices
- Editing the newly arrived revised "facts"
September
- Regular team meetings
- Looking for sponsors
- Contacting the media for outreach purposes
- Designing our team t-shirts
Wetlab
- Digesting samples
- Gel extraction purification
- PCR purification
- Nanodrop test
- Ligation
- Transformation
- Plating
- Colony picking and PCR
- Gel analysis
Drylab
- Working on the pharmacokynetics model last details
Human Practices
- Editing the newly arrived revised "facts"
- Designing promotional goodies for the Synthetic Facts website
- Regular team meetings
- Looking for sponsors
- Contacting the media for outreach purposes
Wetlab
- Preparation for protein expression
- Preparation of LB medium
- Protein expression
- Making more BioBricks
- Preparation for CEST measurements
- Miniprepping
- PCR and PCR purification
- Gel extraction
- Re-digesting 1G70
- Ligation
Drylab
- Working on the update of the models on the wiki
Human Practices
- Editing the newly arrived revised "facts"
- Getting promotional goodies for the Synthetic Facts website
- Regular team meetings
- Looking for sponsors
- Contacting the media for outreach purposes
- Designing the poster and the presentation
Wetlab
- Transformation and plating
- Colony picking and PCR
- Small cultures
- Miniprepping
- Preparing the BioBricks for shippment to Boston
- Making of samples for sequencing
Drylab
- Working on the update of the models on the wiki
Human Practices
- Editing the newly arrived revised "facts"
- Regular team meetings
- Looking for sponsors
- Contacting the media for outreach purposes
- Designing the poster and the presentation
Wetlab
- Preparing the BioBricks for shipment once more due to an external issue with the shipment of the BioBricks
- Culturing bacteria
- Prepare bacteria for electroporation and electroporation
- Creating small samples
- Preparing LB medium
Drylab
- Working on the update of the models on the wiki
Human Practices
- Editing the newly arrived revised "facts"
- Regular team meetings
- Contacting the media for outreach purposes
- Adding the final touches to the poster and the presentation
- Adding final details to the wiki
- Adding last details on the judging form
Wetlab
- Protein purification
- SDS analysis
- Fluorescence analysis
- Filling in the characterization forms from our BioBricks on the registry
Drylab
- Double checking modeling content on the wiki
Human Practices
- Editing the newly arrived revised "facts"
- Making sure all the goodies for the Jamboree are good to go