Team:TU-Delft/LabWork

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<h2 align="center">Labwork</h2>
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<p align="justify">Modeling will play an important role in the iGEM project. Our total system is rather complex and requires careful tuning in order for it to work. This tuning cannot be done in the lab due to time constraints, so the modeling will be used to derive conditions on strengths of promoters and binding sites in order for the system to work. This way the correct implementation can be done in the lab, saving effort and time.
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The modeling will first focus on the timer, then the SUMO fusion and finally the kill switch. These parts will be introduced below and details on these parts will be added later on in the project.
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<b><font color="#330000" size="3">General concepts</font></b>
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<p align="justify" margin: 20px >The most important aspect here is the modeling of the ‘time delay’: What is the difference in time between inducing and the output. In our case we have two outputs, a small delay must also be present between those two.
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At the moment only one output is currently modeled, the ULP-1 protease.  Results of this simulation are shown in Figure 1, here the time delay is about …  minutes. Next to add is the interaction with the peptide tagged with SUMO and the ULP-1 protease. The second output will be added later (the kill switch cassette).
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<b><font color="#330000" size="3">Plasmic constructs</font></b>
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Plasmid constructs
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<b><font color="#330000" size="3">Experiments</font></b>
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EXPERIMENTS
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<b><font color="#330000" size="3">Protocols</font></b>
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PROTOCOLS
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Revision as of 09:30, 11 July 2013

Labwork


Modeling will play an important role in the iGEM project. Our total system is rather complex and requires careful tuning in order for it to work. This tuning cannot be done in the lab due to time constraints, so the modeling will be used to derive conditions on strengths of promoters and binding sites in order for the system to work. This way the correct implementation can be done in the lab, saving effort and time. The modeling will first focus on the timer, then the SUMO fusion and finally the kill switch. These parts will be introduced below and details on these parts will be added later on in the project.


General concepts

The most important aspect here is the modeling of the ‘time delay’: What is the difference in time between inducing and the output. In our case we have two outputs, a small delay must also be present between those two.


At the moment only one output is currently modeled, the ULP-1 protease. Results of this simulation are shown in Figure 1, here the time delay is about … minutes. Next to add is the interaction with the peptide tagged with SUMO and the ULP-1 protease. The second output will be added later (the kill switch cassette).


Plasmic constructs

Plasmid constructs

Experiments

EXPERIMENTS

Protocols

PROTOCOLS

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