Team:TU-Delft/LabWork

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<p align="justify">Modeling will play an important role in the iGEM project. Our total system is rather complex and requires careful tuning in order for it to work. This tuning cannot be done in the lab due to time constraints, so the modeling will be used to derive conditions on strengths of promoters and binding sites in order for the system to work. This way the correct implementation can be done in the lab, saving effort and time.
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The modeling will first focus on the timer, then the SUMO fusion and finally the kill switch. These parts will be introduced below and details on these parts will be added later on in the project.
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The lab work contains:
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<b><font color="#330000" size="3">General concepts</font></b>  
<b><font color="#330000" size="3">General concepts</font></b>  
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<p align="justify" margin: 20px >The most important aspect here is the modeling of the ‘time delay’: What is the difference in time between inducing and the output. In our case we have two outputs, a small delay must also be present between those two.
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The general concepts needed to understand this project are described in this page.
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<img src="https://static.igem.org/mediawiki/2013/1/1a/Timer_simple.png" height=300px>
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At the moment only one output is currently modeled, the ULP-1 protease.  Results of this simulation are shown in Figure 1, here the time delay is about …  minutes. Next to add is the interaction with the peptide tagged with SUMO and the ULP-1 protease. The second output will be added later (the kill switch cassette).
 
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<b><font color="#330000" size="3">Plasmic constructs</font></b>  
<b><font color="#330000" size="3">Plasmic constructs</font></b>  
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Plasmid constructs
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Plasmid constructs contains the description of
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<b><font color="#330000" size="3">Experiments</font></b>  
<b><font color="#330000" size="3">Experiments</font></b>  
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EXPERIMENTS
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EXPERIMENTS  
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Latest revision as of 09:57, 11 July 2013

Labwork


The lab work contains:


General concepts

The general concepts needed to understand this project are described in this page.


Plasmic constructs

Plasmid constructs contains the description of

Experiments

EXPERIMENTS

Protocols

PROTOCOLS

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