Team:INSA Toulouse/contenu/project/overview

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  <h1 class="title1">Project</h1>
 
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   <h3 class="title3">Why <i>E.calculus ?</i></h3>
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   <h2 class="title2">Why <i>E.calculus ?</i></h2>
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When we first made brainstorming trying to define our iGEM project for this year, we realized that addition is present everywhere in living world, as example in many intracellular regulations and controls, or even in control of metabolic pathways. So we decided to create a n-bit bacterial full adder, which could be adjustable by the user, able to transmit a carry if needed. Considering every iGEM projects already based on the adder concept, and using the all new systems of logic recombinatorial gates, we aim to create a robust and strong device able to be implemented on bacterial chassis. We also want to prove (demonstrate / reveal / show) in this project how recombinatorial gates could be considered as the easiest and strongest way to build genetic logic gates, which could be used together in the creation of many genetic devices.</p>
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During our brainstorming meetings to decide what would be our iGEM project, many ideas on building artificial regulations came out. We realized that most biological regulation systems perform highly specific implicit operations (greater than, lower than, if, etc.). However, none of them are prone to execute real mathematical operations. We therefore decided to create a n-bit bacterial full adder which could be operated by the user and able to transmit a carry if needed. Considering that some of the previous iGEM projects tackled the bacterial adder concept, we envisioned a new strategy using recombination-based logic gates. The most important achievements were to demonstrate that recombination-based switches represent simple yet highly efficient genetic logic gates modules that can be further integrated into more complex genetic devices. Besides the full adder idea, these genetic devices should lead to substantial progresses in the design of artificially regulated synthetic pathways. </p>
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   <h3 class="title3">From TI83+ to <i>E.calculus</i></h3>
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   <p class="texte">An electronic full-adder consists of the association of 5 logic gates (2 XOR gates, 2 AND gates, and 1 OR gate) which are able to account for 3 input signals (2 input bits and the last stage carry) and give the right answer to the user. (link to “full adder” part)
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   <p class="texte">An electronic full-adder consists of the association of 5 logic gates (2 XOR gates, 2 AND gates, and 1 OR gate) which are able to receive 3 input signals (2 input bits and the last stage carry) and give the right answer to the user. <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/full-adder">(View "Full Adder" Part)</a></li>
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   With <i>E. calculus</i> we tried to link the various logic gate genetic devices to:
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       <li>A semi-artificial input system converting 0 and 1 input signals (absence and presence of blue and red lights) into biological signals.<a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">(View "Input" Part)</a>
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       <li>The production and diffusion of a bacterial signalling molecule, acyl homoserine lactone (AHL) for the carry, transmitting the information of from one cell to the other <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/carry">(View "Carry" Part)</a>
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       <li>An easily readable result of the various operations consisting of a clearly visible output pigment: the Red Fluorescent Protein (RFP) <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/output">(View "Output" Part)</a>
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Latest revision as of 15:28, 4 October 2013

logo


Overview

Why E.calculus ?

During our brainstorming meetings to decide what would be our iGEM project, many ideas on building artificial regulations came out. We realized that most biological regulation systems perform highly specific implicit operations (greater than, lower than, if, etc.). However, none of them are prone to execute real mathematical operations. We therefore decided to create a n-bit bacterial full adder which could be operated by the user and able to transmit a carry if needed. Considering that some of the previous iGEM projects tackled the bacterial adder concept, we envisioned a new strategy using recombination-based logic gates. The most important achievements were to demonstrate that recombination-based switches represent simple yet highly efficient genetic logic gates modules that can be further integrated into more complex genetic devices. Besides the full adder idea, these genetic devices should lead to substantial progresses in the design of artificially regulated synthetic pathways.

From TI83+ to E. calculus

An electronic full-adder consists of the association of 5 logic gates (2 XOR gates, 2 AND gates, and 1 OR gate) which are able to receive 3 input signals (2 input bits and the last stage carry) and give the right answer to the user. (View "Full Adder" Part)

With E. calculus we tried to link the various logic gate genetic devices to:

  • A semi-artificial input system converting 0 and 1 input signals (absence and presence of blue and red lights) into biological signals.(View "Input" Part)
  • The production and diffusion of a bacterial signalling molecule, acyl homoserine lactone (AHL) for the carry, transmitting the information of from one cell to the other (View "Carry" Part)
  • An easily readable result of the various operations consisting of a clearly visible output pigment: the Red Fluorescent Protein (RFP) (View "Output" Part)

Retrieved from "http://2013.igem.org/Team:INSA_Toulouse/contenu/project/overview"