Team:Evry/Inverter

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<b>Fig</b> Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed.
<b>Fig</b> Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed.
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<h1>1<sup>st</sup> Plasmid with LacI </h1>
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<div align='center'><img src="https://static.igem.org/mediawiki/2013/c/c8/P1-Lac.png" width="75%"/></div>
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<table cellpadding="10" cellspacing="0" align='center' border="1">
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  <tr>
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    <th align="center">
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        <b>NAME</b>
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    </th>
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    <th align="center">
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      <b>FIGURE</b>
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    </th>
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    <th align="center">
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        <b>PARTICULARITY</b>
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  <tr>
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    <td align="center">
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      <p>
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        Promoter
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      </p>
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    </td>
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    <td align="center">
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      <img src="https://static.igem.org/mediawiki/2013/6/6c/Part1.1.png" width="250px"/>
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    <td align="center">
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      <p>
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        Comment...
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  <tr>
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    <td align="center">
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      <p>
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        LacI LVA
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      </p>
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    </td>
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    <td align="center">
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      <img src="https://static.igem.org/mediawiki/2013/0/0b/Part1.5.png" width="300px"/>
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    <td align="center">
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      <p>
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        Comment...
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    <td align="center">
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      <p>
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        Terminator
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      </p>
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    </td>
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    <td align="center">
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      <img src="https://static.igem.org/mediawiki/2013/a/ac/Part1.3.png" width="170px"/>
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    <td align="center">
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      <p>
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        Comment...
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  <tr>
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    <td align="center">
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      <p>
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        Plasmid
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      </p>
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    </td>
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    <td align="center">
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      <img src="https://static.igem.org/mediawiki/2013/d/d3/Part1.4.png" width="310px"/>
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    </td>
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    <td align="center">
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      <p>
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        Comment...
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      </p>
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  </tr>
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Revision as of 16:10, 4 October 2013

Iron coli project

Fur Inverter

Normally, the Ferric Uptake Regulator (Fur) binds iron to repress transcription of its target genes. However, we needed a system that activates gene expression in response to iron. We thus constructed a "genetic invertor" system that reverses the Fur-regulatory system so that it indirectly activates gene expression in response to iron (Fig 1). This system consists of a Fur-regulated lacI gene and a LacI regulated reporter gene. When Fur binds iron, it represses expression of the LacI repressor which, in turn, permits expression of the reporter gene. Thus, reporter expression is positively correlated with iron concentration.

Fig Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed.

1st Plasmid with LacI

NAME FIGURE PARTICULARITY

Promoter

Comment...

LacI LVA

Comment...

Terminator

Comment...

Plasmid

Comment...

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