Team:INSA Toulouse/contenu/project/biological construction

From 2013.igem.org

(Difference between revisions)
 
(7 intermediate revisions not shown)
Line 43: Line 43:
   .imgcontent{margin: 0 0 45px 0; border: 1px solid #e5e6e6;}
   .imgcontent{margin: 0 0 45px 0; border: 1px solid #e5e6e6;}
 +
  .imgcenter{display: block; margin-left: auto;margin-right: auto; border: 1px solid #e5e6e6}
   .tablecontent{
   .tablecontent{
Line 78: Line 79:
   <h1 class="title1">Biological Modules</h1>
   <h1 class="title1">Biological Modules</h1>
-
   <p class="texte">The first question we had to face for the <i>E.calculus</i> project was: “how can we transpose an electronic device into a reasonable biological system?”
+
   <p class="texte">The first question we had to face for the <i>E.calculus</i> project was the transposition of an electronic device into a reasonable biological system.
-
<br>The diagramm of an electronic full adder can be divided into two independant parts : Input and Output signals (A, B, C<sub>in</sub>, S, C<sub>out</sub>) and logic gates. Indeed, logic gates will ever be the same, but signals must be adaptable to considered applications and microorganisms.</p>
+
<br>The diagram of an electronic full adder (see below) can be divided into three independant parts: Input and Output signals (A, B, C<sub>in</sub>, S, C<sub>out</sub>) and logic gates (XOR, AND, OR). The rationale for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.</p>
    
    
-
   <img src="https://static.igem.org/mediawiki/2013/2/2c/Full-adder.png" class="imgcontent" />
+
   <img src="https://static.igem.org/mediawiki/2013/2/2c/Full-adder.png" class="imgcenter" />
 +
  <br>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">Input</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">Input</span></h2>
-
   <p class="texte">For the input, it was needed to use a signal that can easily represents an “ON and OFF” switch. The use of lights to represent the inputs was our first idea : a blue and a red light.</a></p>
+
   <p class="texte">For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).</a></p>
 +
 
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/output">Output</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/output">Output</span></h2>
-
   <p class="texte">The output needed to be a signal that can be easily seen without any divice, something visual like a color.</a></p>
+
   <p class="texte">The output needed to be a signal that can be easily seen without any complicated device or apparatus, something visual like the color of the organism bearing the full adder.</a></p>
 +
 
 +
 
 +
  <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/carry">Carry</span></h2>
 +
  <p class="texte">The carry (C<sub>in</sub> and C<sub>out</sub>), belongs to both the input and output modules. We thought of a molecule that could transmit a message from one colony to the other. </a></p>
-
  <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/riboregulation">Riboregulation System</span></h2>
 
-
  <p class="texte">In order to better control the expression of the recombinases, a ribo regulation system has been add before the recombinases genes.</a></p>
 
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/logic_gates">Logic Gates</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/logic_gates">Logic Gates</span></h2>
-
   <p class="texte">An electronic full adder is composed of 5 logic gates (2 XOR, 2 AND and 1 OR). To transpose these logic gates into biological gates, two publications published this year (2013) inspired us.</a></p>
+
   <p class="texte">An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination-based logic gates. They inspired us and are the basis of our work.</a></p>
-
  <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/carry">Carry</span></h2>
 
-
  <p class="texte">To represent the carry, a molecule that can transmit a message from one colony to an other was essential. </a></p>
 
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/full_adder">Full Adder</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/full_adder">Full Adder</span></h2>
-
   <p class="texte">With a logic diagram, a full adder can be illustrated with 5 logic gates (2 XOR, 2 AND and 1 OR) A and B represent the two operands and Cin and Cout the carries. In our biological system, this diagramm represents the input part.</a></p>
+
   <p class="texte">The full adder is the integration of the signals (input, ouput, carry) with the logic gates. Its biological description can be found here.</a></p>

Latest revision as of 16:26, 4 October 2013

logo


Biological Modules

The first question we had to face for the E.calculus project was the transposition of an electronic device into a reasonable biological system.
The diagram of an electronic full adder (see below) can be divided into three independant parts: Input and Output signals (A, B, Cin, S, Cout) and logic gates (XOR, AND, OR). The rationale for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.


Input

For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).

Output

The output needed to be a signal that can be easily seen without any complicated device or apparatus, something visual like the color of the organism bearing the full adder.

Carry

The carry (Cin and Cout), belongs to both the input and output modules. We thought of a molecule that could transmit a message from one colony to the other.

Logic Gates

An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination-based logic gates. They inspired us and are the basis of our work.

Full Adder

The full adder is the integration of the signals (input, ouput, carry) with the logic gates. Its biological description can be found here.

Retrieved from "http://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction"