Team:TU-Munich/Modeling/Overview

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===Enzyme Kinetics===
===Enzyme Kinetics===
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For the effective implementation of our filter system it is essential to analyse the enzymatic activity of our effectors. To do this in a sophisticated fashion, we carried out a series of ''in vitro'' assays with different concentrations. Using this experimental data we fitted the kinetic parameters for the enzymatically catalized conversion reaction and carried out rigorous uncertainty analysis to assess the reliability of the fitted parameters.
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For the effective implementation of our filter system it is essential to analyse the enzymatic activity of our effectors. Using this experimental data we fitted the kinetic parameters for the enzymatically catalized conversion reaction and carried out rigorous uncertainty analysis to assess the reliability of the fitted parameters.
([https://2013.igem.org/Team:TU-Munich/Modeling/Enzyme Read More])  
([https://2013.igem.org/Team:TU-Munich/Modeling/Enzyme Read More])  
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Revision as of 18:20, 4 October 2013


Modeling Overview

Protein Predictions

For the immobilisation of effectors on the cell membrane, we needed to design a transmembrane domain. Using several bioinformatic methods we identified the transmembrane region of the SERK receptor which we later used as starting point for our constructs. (Read More)

Enzyme Kinetics

For the effective implementation of our filter system it is essential to analyse the enzymatic activity of our effectors. Using this experimental data we fitted the kinetic parameters for the enzymatically catalized conversion reaction and carried out rigorous uncertainty analysis to assess the reliability of the fitted parameters. (Read More)

Kill Switch

During the planning stage of our project, we had several different ideas on how to efficiently implement a kill-switch in our moss. In this section of the wiki we documented our mathematical train of thought that eventually led us to our final design. (Read More)

Filter Model

The filter model is aimed to simulate different remediation scenarios and should be used to calculate the perfectly fitting conditions of our Physco filter, referring to the needs of the environment. (Read More)

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