Team:INSA Toulouse/contenu/safety/iGEM safety

From 2013.igem.org

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   <h1 class="title1">iGEM Safety</h1>
   <h1 class="title1">iGEM Safety</h1>
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  <h2 class="title2">Abstract</h2>
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  <p class="texte2"> Safety forms were approved on September 29, 2013 by the iGEM Safety Committee.</p>
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   <p class="texte">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut et dolor turpis. Suspendisse interdum, dolor eu  ultricies dignissim, arcu sapien placerat quam, ut bibendum lorem nibh id magna. Nunc condimentum lectus at diam tempus sodales. Nullam scelerisque auctor tellus, nec auctor ante elementum sit amet. Aenean venenatis velit eget porttitor laoreet. Aliquam aliquam libero in ante imperdiet vehicula. Donec nibh urna, tincidunt quis condimentum at, tempus sed sapien. Nunc elit dui, dignissim tincidunt mi non, ultrices ultricies erat.</p>
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   <h2 class="title2">Chassis Organism</h2>
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  <img src="https://static.igem.org/mediawiki/2013/b/b7/Insa-toulouse2013-phototestpage.jpg" class="imgcontent" />
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  <p class="texteleft"><span class="spantitle">lorem ipsum</span></br>
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  <table class="tablecontent">
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<br>Mauris ac suscipit erat, sit amet blandit lectus. Fusce placerat, lectus at suscipit viverra, lorem lorem mattis turpis, id pharetra velit nunc vitae velit. Etiam ultrices aliquam ligula, sed ultrices nibh vulputate non. Ut dapibus arcu luctus, suscipit urna et, imperdiet magna. Curabitur tristique sed diam non elementum. Sed lectus urna, consequat quis porta eu, ultrices in nunc. Aenean vitae elit neque.<p>
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  <p class="texteright"><span class="spantitle">lorem ipsum</span></br>
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<tr style="background-color:#20a8da; height:50px; color:#ffffff;" >
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<br>Mauris ac suscipit erat, sit amet blandit lectus. Fusce placerat, lectus at suscipit viverra, lorem lorem mattis turpis, id pharetra velit nunc vitae velit. Etiam ultrices aliquam ligula, sed ultrices nibh vulputate non. Ut dapibus arcu luctus, suscipit urna et, imperdiet magna. Curabitur tristique sed diam non elementum. Sed lectus urna, consequat quis porta eu, ultrices in nunc. Aenean vitae elit neque.</p>
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<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;">Species</td>
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<td style="border-bottom:4px solid #e5e6e6;">Strain n°/name</td>
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<div class="clear"></div>
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<td style="border-bottom:4px solid #e5e6e6;">Risk Group</td>
-
 
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<td style="border-bottom:4px solid #e5e6e6;">Risk group source link</td>
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  <div class="list">
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<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;">Disease risk to humans? If so, which disease?</td>
-
   
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</tr>
-
    <ul class="arrow">
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<tr>
-
      <li>Quisque at massa ipsum</li>
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<td style="border-right:1px solid #e5e6e6;"><i>E.coli</i> (K12)</td>
-
      <li>Maecenas a lorem augue, egestas </li>
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<td style="border-right:1px solid #e5e6e6;">DH5-alpha</td>
-
      <li>Cras vitae felis at lacus ele </li>
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<td style="border-right:1px solid #e5e6e6;">1</td>
-
      <li>Etiam auctor diam pellentesque </li>
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<td style="border-right:1px solid #e5e6e6;"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli" target="_blank">Link</a></td>
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      <li>Nulla ac massa at dolor </li>
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<td>Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
-
    </ul>
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</tr>
-
 
+
<tr >
-
  </div>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><i>E. coli</i> (K12)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">DH5-1</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli" target="_blank">Link</a></td>
 +
<td style="border-top:1px solid #e5e6e6;">Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
 +
</tr>
 +
<tr >
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><i>E. coli</i> (K12)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">MG1655</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli" target="_blank">Link</a></td>
 +
<td style="border-top:1px solid #e5e6e6;">Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
 +
</tr>
 +
<tr >
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><i>E. coli</i> (K12)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">BL21-DE3</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli" target="_blank">Link</a></td>
 +
<td style="border-top:1px solid #e5e6e6;">Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
 +
</tr>
 +
<tr >
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><i>E. coli</i> (K12)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">XL1-Blue</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;"><a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=&species=coli" target="_blank">Link</a></td>
 +
<td style="border-top:1px solid #e5e6e6;">Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.</td>
 +
</tr>
 +
<tr>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;"><i>Chromobacterium violaceum</i></td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">CV026</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">2</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"> <a href="http://www.absa.org/riskgroups/bacteriasearch.php?genus=Chromobacterium" target="_blank">Link</a></td>
 +
<td style="border-top:1px solid #e5e6e6;">Yes. Pathogen for humans and vertebrates, but normally no transmission between the two host groups. It rarely infects humans, but when it does it causes skin lesions, sepsis, and liver abscesses that may be fatal.</td>
 +
</tr>
 +
</table>
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  <div class="list">
 
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    <ul class="circlearrow">
 
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      <li>Quisque at massa ipsum</li>
 
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      <li>Maecenas a lorem augue, egestas </li>
 
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      <li>Cras vitae felis at lacus ele </li>
 
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      <li>Etiam auctor diam pellentesque </li>
 
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      <li>Nulla ac massa at dolor </li>
 
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  </div>
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  <h2 class="title2">New and/or Modified Coding Regions</h2>
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  <div class="list">
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    <ul class="arrow">
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      <li>Quisque at massa ipsum</li>
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-
      <li>Maecenas a lorem augue, egestas </li>
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-
      <li>Cras vitae felis at lacus ele </li>
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-
      <li>Etiam auctor diam pellentesque </li>
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-
      <li>Nulla ac massa at dolor </li>
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    </ul>
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-
 
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  </div>
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<div class="clear"></div>
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  <h3 class="title3">TITRE 3 lorem ipsum</h3>
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  <p class="texte">Lorem ipsum dolor sit amet, consectetur adipiscing elit. Ut et dolor turpis. Suspendisse interdum, dolor eu  ultricies dignissim, arcu sapien placerat quam, ut bibendum lorem nibh id magna. Nunc condimentum lectus at diam tempus sodales. Nullam scelerisque auctor tellus, nec auctor ante elementum sit amet. Aenean venenatis velit eget porttitor laoreet. Aliquam aliquam libero in ante imperdiet vehicula. Donec nibh urna, tincidunt quis condimentum at, tempus sed sapien. Nunc elit dui, dignissim tincidunt mi non, ultrices ultricies erat.</p>
+
   <table class="tablecontent">
   <table class="tablecontent">
<tr style="background-color:#20a8da; height:50px; color:#ffffff;" >
<tr style="background-color:#20a8da; height:50px; color:#ffffff;" >
-
<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;">TR  Title</td>
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<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;">Part number</td>
-
<td style="border-bottom:4px solid #e5e6e6;">TR  Title</td>
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<td style="border-bottom:4px solid #e5e6e6;">Description</td>
-
<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;">TR  Title</td>
+
<td style="border-bottom:4px solid #e5e6e6;">Where did we get the physical DNA for this part</td>
 +
<td style="border-bottom:4px solid #e5e6e6;">What species does this part originally come from?</td>
 +
<td style="border-bottom:4px solid #e5e6e6;">What is the Risk Group of the species?</td>
 +
<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;">What is the function of this part, in its parent species?</td>
</tr>
</tr>
<tr>
<tr>
-
<td style="border-right:1px solid #e5e6e6;">Quisque</td>
+
<td style="border-right:1px solid #e5e6e6;font-size:12px;">BBa_K1132000</td>
-
<td style="border-right:1px solid #e5e6e6;">Maecenas a lorem augue</td>
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<td style="border-right:1px solid #e5e6e6;">T7 Polymerase</td>
-
<td>Maecenas a lorem augue</td>
+
<td style="border-right:1px solid #e5e6e6;">PCR extraction from genomic DNA of <i>E. coli</i> K12 BL21-DE3</td>
 +
<td style="border-right:1px solid #e5e6e6;">Bacteriophage T7</td>
 +
<td style="border-right:1px solid #e5e6e6;">1</td>
 +
<td>RNA Polymerase</td>
</tr>
</tr>
-
<tr style="border-top:1px solid #e5e6e6">
+
<tr >
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132001</td>
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
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<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Tp901 sites<br>
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
Bxb1 sites<br>
 +
pT7<br>
 +
BBa_B0015<br>
 +
Bba_J61048</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Bacteriophage TP901<br>
 +
Bacteriophage BXB1<br>
 +
Artificial sequence <a href="http://www.ncbi.nlm.nih.gov/pubmed/23539178" target="_blank">(Bonnet et al. 2013)</a><br>
 +
Double terminator<br>
 +
T1 terminator</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Recombinase site<br>
 +
Recombinase site<br>
 +
Promoter region<br>
 +
Transcription terminator<br>
 +
Transcription terminator</td>
 +
</tr>
 +
<tr >
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132002</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Tp901 sites<br>
 +
Bxb1 sites<br>
 +
pT7<br>
 +
BBa_B0015</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Bacteriophage TP901<br>
 +
Bacteriophage BXB1<br>
 +
Artificial sequence <a href="http://www.ncbi.nlm.nih.gov/pubmed/23539178" target="_blank">(Bonnet et al. 2013)</a><br>
 +
Double terminator</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Recombinase site<br>
 +
Recombinase site<br>
 +
Promoter region<br>
 +
Transcription terminator</td>
 +
</tr>
 +
<tr >
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132003</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">FimE sites<br>
 +
PhiC31 sites<br>
 +
BBa_I712074</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">E. coli (K12) FimE<br>
 +
Bacteriophage PhiC31<br>
 +
T7 Promoter</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Recombinase site<br>
 +
Recombinase site<br>
 +
Promoter region</td>
 +
</tr>
 +
<tr >
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132004</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">FimE sites<br>
 +
PhiC31 sites<br>
 +
BBa_I712074</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">E. coli (K12) FimE<br>
 +
Bacteriophage PhiC31<br>
 +
T7 Promoter</td>
 +
<td style="border-right:1px solid #e5e6e6;border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Recombinase site<br>
 +
Recombinase site<br>
 +
Promoter region</td>
</tr>
</tr>
<tr>
<tr>
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132005</td>
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">BBa_R0040<br>
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
riboregulator R0.1<br>
 +
BBa_B0015<br>
 +
BBa_R0065<br>
 +
riboregulator R0.2</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"> TetR promoter<br>
 +
Artificial sequence<br>
 +
Double terminator<br>
 +
Promoter (lambda cI and luxR regulated)<br>
 +
Artificial sequence</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Promoter region<br>
 +
RNA sequence for regulation<br>
 +
Transcription terminator<br>
 +
Promoter region<br>
 +
RNA sequence for regulation</td>
</tr>
</tr>
<tr>
<tr>
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Quisque</td>
+
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132006</td>
-
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">BBa_R0040<br>
-
<td style="border-top:1px solid #e5e6e6;">Maecenas a lorem augue</td>
+
riboregulator R1.1<br>
 +
BBa_B0015<br>
 +
BBa_R0065<br>
 +
riboregulator R1.2</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"> TetR promoter<br>
 +
Artificial sequence<br>
 +
Double terminator<br>
 +
Promoter (lambda cI and luxR regulated)<br>
 +
Artificial sequence</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Promoter region<br>
 +
RNA sequence for regulation<br>
 +
Transcription terminator<br>
 +
Promoter region<br>
 +
RNA sequence for regulation</td>
 +
</tr>
 +
<tr>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132007</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">BBa_R0040<br>
 +
riboregulator R2.1<br>
 +
BBa_B0015<br>
 +
BBa_K592006<br>
 +
riboregulator R2.2</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"> TetR promoter<br>
 +
Artificial sequence<br>
 +
Double terminator<br>
 +
FixK2 promoter<br>
 +
Artificial sequence</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Promoter region<br>
 +
RNA sequence for regulation<br>
 +
Transcription terminator<br>
 +
Promoter region<br>
 +
RNA sequence for regulation</td>
 +
</tr>
 +
<tr>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132008</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">BBa_R0040<br>
 +
riboregulator R4.1<br>
 +
BBa_B0015<br>
 +
BBa_R0082<br>
 +
riboregulator R4.2</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Gene synthesis (GeneCust)</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"> TetR promoter<br>
 +
Artificial sequence<br>
 +
Double terminator<br>
 +
OmpR-controlled promoter<br>
 +
Artificial sequence</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Promoter region<br>
 +
RNA sequence for regulation<br>
 +
Transcription terminator<br>
 +
Promoter region<br>
 +
RNA sequence for regulation</td>
 +
</tr>
 +
<tr>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132009</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">PhiC31 integrase</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">From the plasmid A24 from Suiti Laboratory (MIT)</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">bacteriophage phiC31</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Recombinase</td>
 +
</tr>
 +
<tr>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;font-size:12px;">BBa_K1132010</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">Tp901 integrase</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">From the parts KC529324 from Jerome Bonnet</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">bacteriophage Tp901</td>
 +
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;">1</td>
 +
<td style="border-top:1px solid #e5e6e6;">Recombinase</td>
</tr>
</tr>
</table>
</table>
 +
 +
 
 +
<div class="clear"></div>
 +
 +
  <h3 class="title3">Risks</h3>
 +
 +
  <p class="texte">The two chassis strains (<i>E. coli, C. violaceum</i>) are potential pathogenes for humans. For <i>E. coli</i>, the risks are minimal, but good laboratory practises were applied to avoid possible contaminations. For <i>C. violaceum</i>, all experiments involving this strain will be performed in a P2 facility and under the supervision of a member of the permanent staff who is the manager of the P2 facility.<br>
 +
There is no environmental risks.
 +
<br><br>
 +
<i>C. violaceum</i> is used only for parts characterisation and is not part of the original project. For the project itself, only the <i>E. coli</i> K12 is used and the potential risks of converting the project into a commercial product are already assessed for industrial purposes.<br>
 +
In the potential case where some parts should be used for other purposes than research, precautions shoud be taken to assess the safety of these microorganisms. No parts will be introduced into <i>C. violaceum</i>, avoiding possible antibiotic resistance of the strain.<br><br>
 +
All students receive a 2 hours safety course provided by the biosafety commitee executive. All of them were required to take a test at the end, and all of them passed.<br>
 +
There is an institutional biosafety commitee. The project was approved by several teachers of the institute.<br><br>
 +
The Biosafety level of our lab is 2. The institute has a P2 room fully equiped for manipulating level 2 risk group organisms.<br>
 +
The chassis organism risk group is 1. However, we also use a level 2 test chassis that is only manipulated in the P2 facility.</p>
 +

Latest revision as of 20:22, 4 October 2013

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iGEM Safety

Safety forms were approved on September 29, 2013 by the iGEM Safety Committee.

Chassis Organism

Species Strain n°/name Risk Group Risk group source link Disease risk to humans? If so, which disease?
E.coli (K12) DH5-alpha 1 Link Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.
E. coli (K12) DH5-1 1 Link Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.
E. coli (K12) MG1655 1 Link Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.
E. coli (K12) BL21-DE3 1 Link Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.
E. coli (K12) XL1-Blue 1 Link Yes. May cause irritation to skin, eyes, and respiratory tract, may affect kidneys.
Chromobacterium violaceum CV026 2 Link Yes. Pathogen for humans and vertebrates, but normally no transmission between the two host groups. It rarely infects humans, but when it does it causes skin lesions, sepsis, and liver abscesses that may be fatal.

New and/or Modified Coding Regions

Part number Description Where did we get the physical DNA for this part What species does this part originally come from? What is the Risk Group of the species? What is the function of this part, in its parent species?
BBa_K1132000 T7 Polymerase PCR extraction from genomic DNA of E. coli K12 BL21-DE3 Bacteriophage T7 1 RNA Polymerase
BBa_K1132001 Tp901 sites
Bxb1 sites
pT7
BBa_B0015
Bba_J61048		 Gene synthesis (GeneCust) Bacteriophage TP901
Bacteriophage BXB1
Artificial sequence (Bonnet et al. 2013)
Double terminator
T1 terminator		 1 Recombinase site
Recombinase site
Promoter region
Transcription terminator
Transcription terminator
BBa_K1132002 Tp901 sites
Bxb1 sites
pT7
BBa_B0015		 Gene synthesis (GeneCust) Bacteriophage TP901
Bacteriophage BXB1
Artificial sequence (Bonnet et al. 2013)
Double terminator		 1 Recombinase site
Recombinase site
Promoter region
Transcription terminator
BBa_K1132003 FimE sites
PhiC31 sites
BBa_I712074		 Gene synthesis (GeneCust) E. coli (K12) FimE
Bacteriophage PhiC31
T7 Promoter		 1 Recombinase site
Recombinase site
Promoter region
BBa_K1132004 FimE sites
PhiC31 sites
BBa_I712074		 Gene synthesis (GeneCust) E. coli (K12) FimE
Bacteriophage PhiC31
T7 Promoter		 1 Recombinase site
Recombinase site
Promoter region
BBa_K1132005 BBa_R0040
riboregulator R0.1
BBa_B0015
BBa_R0065
riboregulator R0.2		 Gene synthesis (GeneCust) TetR promoter
Artificial sequence
Double terminator
Promoter (lambda cI and luxR regulated)
Artificial sequence		 1 Promoter region
RNA sequence for regulation
Transcription terminator
Promoter region
RNA sequence for regulation
BBa_K1132006 BBa_R0040
riboregulator R1.1
BBa_B0015
BBa_R0065
riboregulator R1.2		 Gene synthesis (GeneCust) TetR promoter
Artificial sequence
Double terminator
Promoter (lambda cI and luxR regulated)
Artificial sequence		 1 Promoter region
RNA sequence for regulation
Transcription terminator
Promoter region
RNA sequence for regulation
BBa_K1132007 BBa_R0040
riboregulator R2.1
BBa_B0015
BBa_K592006
riboregulator R2.2		 Gene synthesis (GeneCust) TetR promoter
Artificial sequence
Double terminator
FixK2 promoter
Artificial sequence		 1 Promoter region
RNA sequence for regulation
Transcription terminator
Promoter region
RNA sequence for regulation
BBa_K1132008 BBa_R0040
riboregulator R4.1
BBa_B0015
BBa_R0082
riboregulator R4.2		 Gene synthesis (GeneCust) TetR promoter
Artificial sequence
Double terminator
OmpR-controlled promoter
Artificial sequence		 1 Promoter region
RNA sequence for regulation
Transcription terminator
Promoter region
RNA sequence for regulation
BBa_K1132009 PhiC31 integrase From the plasmid A24 from Suiti Laboratory (MIT) bacteriophage phiC31 1 Recombinase
BBa_K1132010 Tp901 integrase From the parts KC529324 from Jerome Bonnet bacteriophage Tp901 1 Recombinase

Risks

The two chassis strains (E. coli, C. violaceum) are potential pathogenes for humans. For E. coli, the risks are minimal, but good laboratory practises were applied to avoid possible contaminations. For C. violaceum, all experiments involving this strain will be performed in a P2 facility and under the supervision of a member of the permanent staff who is the manager of the P2 facility.
There is no environmental risks.

C. violaceum is used only for parts characterisation and is not part of the original project. For the project itself, only the E. coli K12 is used and the potential risks of converting the project into a commercial product are already assessed for industrial purposes.
In the potential case where some parts should be used for other purposes than research, precautions shoud be taken to assess the safety of these microorganisms. No parts will be introduced into C. violaceum, avoiding possible antibiotic resistance of the strain.

All students receive a 2 hours safety course provided by the biosafety commitee executive. All of them were required to take a test at the end, and all of them passed.
There is an institutional biosafety commitee. The project was approved by several teachers of the institute.

The Biosafety level of our lab is 2. The institute has a P2 room fully equiped for manipulating level 2 risk group organisms.
The chassis organism risk group is 1. However, we also use a level 2 test chassis that is only manipulated in the P2 facility.

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