Iron Chelator

First strategy for enterobactin biosynthesis

Here we present Fig 1 and 2 our constructions which contain each three Lac I regulated enterobactin synthesis genes. Escherichia coli naturally have those genes into a single operon but due to their important lenghts, we decided to divide them into two indivudual constructions in order to make the cloning easier.

Fig 1 First construction containing the Lac I regulated enterobactin synthesis genes Ent A, Ent D and Ent F. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.

Fig 2 Second construction containing the Lac I regulated enterobactin synthesis genes Ent B, Ent C and Ent E. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.

NAME	FIGURE	Description
Lac promoter		Lac Promoter
RBS + EntA		First gene required for enterobactin sythesis
RBS + EntB		Second gene required for enterobactin sythesis
RBS + EntC		Third gene required for enterobactin sythesis
RBS + EntD		Fourth gene required for enterobactin sythesis
RBS + EntE		Fifth gene required for enterobactin sythesis
RBS + EntF		Sixth gene required for enterobactin synthesis
Terminator		Transcription Stop signal
Plasmid		Backbone with ampicillin resistance

Table 1. Genetic elements used to produce the enterobactin siderophore.

Second strategy for enterobactin biosynthesis

Figure 1: Légende ici.

Future caracterisation of the construction

Even though we tried to simplify the cloning, we did not succeed to obtain our two constructions. Thus, we have conceived new cloning approaches.