Team:Paris Saclay/Notebook/September/26

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{{Team:Paris_Saclay/incl_debut_generique}}
{{Team:Paris_Saclay/incl_debut_generique}}
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='''Notebook : September 12'''=
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='''Notebook : September 26'''=
=='''Lab work'''==
=='''Lab work'''==
===''' Paris-Saclay PCR'''===
===''' Paris-Saclay PCR'''===
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Damir
We tested our PCR machine and it works as expected.
We tested our PCR machine and it works as expected.
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Used quantities :
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* pIMI018 (DNA) : 4µL
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* Primer aacF (10 µM) : 10µL
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* Primer aacR (10 µM) : 10µL
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* dXTP : 2 µL
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* Buffer 10x : 10µL
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* Q solution : 20µL
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* Taq (Qiagen : 0.8µL
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* H2O : 44µL
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[[File:PsPSPCR.jpg|400px]]
{|
{|
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*Well 4 : PCR  product amplified with the PSPCR without oil in the well, 15µl
*Well 4 : PCR  product amplified with the PSPCR without oil in the well, 15µl
*Well 5 : Control PCR  product amplified with a commercial thermal cycler,  5µl
*Well 5 : Control PCR  product amplified with a commercial thermal cycler,  5µl
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|}
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{| border="1" align="center"
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|[[Team:Paris Saclay/Notebook/September/14|<big>Previous day</big>]]
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|[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]]
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|}
|}
{{Team:Paris_Saclay/incl_fin}}
{{Team:Paris_Saclay/incl_fin}}

Latest revision as of 21:53, 4 October 2013

Navigation Home Team Project Labwork Notebook Human practices

Notebook : September 26

Lab work

Paris-Saclay PCR

Damir

We tested our PCR machine and it works as expected.

Used quantities :

  • pIMI018 (DNA) : 4µL
  • Primer aacF (10 µM) : 10µL
  • Primer aacR (10 µM) : 10µL
  • dXTP : 2 µL
  • Buffer 10x : 10µL
  • Q solution : 20µL
  • Taq (Qiagen : 0.8µL
  • H2O : 44µL

PsPSPCR.jpg

Pspcr test.png
  • Well 1 : 15µL DNA Ladder
  • Wells 2 & 3 : PCR products amplified with the PS-PCR with oil in the wells, 15µl
  • Well 4 : PCR product amplified with the PSPCR without oil in the well, 15µl
  • Well 5 : Control PCR product amplified with a commercial thermal cycler, 5µl
Previous day Back to calendar

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