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Team:Paris Saclay/Notebook/September/26
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*Well 4 : PCR product amplified with the PSPCR without oil in the well, 15µl | *Well 4 : PCR product amplified with the PSPCR without oil in the well, 15µl | ||
*Well 5 : Control PCR product amplified with a commercial thermal cycler, 5µl | *Well 5 : Control PCR product amplified with a commercial thermal cycler, 5µl | ||
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| + | |[[Team:Paris Saclay/Notebook/September/14|<big>Previous day</big>]] | ||
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| + | |[[Team:Paris_Saclay/Notebook|<big>Back to calendar</big>]] | ||
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Latest revision as of 21:53, 4 October 2013
Notebook : September 26
Lab work
Paris-Saclay PCR
Damir
We tested our PCR machine and it works as expected.
Used quantities :
- pIMI018 (DNA) : 4µL
- Primer aacF (10 µM) : 10µL
- Primer aacR (10 µM) : 10µL
- dXTP : 2 µL
- Buffer 10x : 10µL
- Q solution : 20µL
- Taq (Qiagen : 0.8µL
- H2O : 44µL
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