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| - | <h3 class="texteleft"><span class="title3">Carry</span></h3> | + | <h3 class="texteright"><span class="title3">Riboswitches</span></h3> |
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| | + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/ribo"><img src="https://static.igem.org/mediawiki/2013/b/b3/Ribo_-_340px.png" class="imgcontentright" /></a> |
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| - | <h3 class="texteright"><span class="title3">General Inducer</span></h3>
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| - | <h3 class="texteleft"><span class="title3">Blue Light Sensor</span></h3> | + | <h3 class="texteleft"><span class="title3">Carry Diffusion Model</span></h3> |
| | + | <h3 class="texteright"><span class="title3">T7 Polymerase</span></h3> |
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| - | <h3 class="texteright"><span class="title3">Red Light Sensor</span></h3>
| + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/carry"><img src="https://static.igem.org/mediawiki/2013/1/1b/Diffusion_results340px.png" class="imgcontentleft" /></a> |
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| - | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/blue_sensor"><img style="width:340px;" src="https://static.igem.org/mediawiki/2013/f/f1/Blue_light_system_notebook_-_340px.png" class="imgcontentleft" /></a>
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| - | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/ribo"><img src="https://static.igem.org/mediawiki/2013/b/b3/Ribo_-_340px.png" class="imgcontentleft" /></a>
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| - | <h3 class="texteright"><span class="title3">MG1655 del(EnvZ) Strain</span></h3>
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| - | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/calendar/MG1655"><img src="https://static.igem.org/mediawiki/2013/1/13/MG1655_-_340px.png" class="imgcontentright" /></a>
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| - | <h3 class="texteright"><span class="title3">T7 Polymerase</span></h3>
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| - | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/ polT7"><img style="width:340px;" src="https://static.igem.org/mediawiki/2013/ 1/ 14/ Pol_t7.jpg" class="imgcontentright" /></a> | + | |
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| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
| + | <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/ results/ red_sensor"> <img src="https://static.igem.org/mediawiki/2013/ 5/ 5b/ Red_light_système_-_340px.png" class=" imgcontentright" /></a> |
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/ notebook/ protocols/charac_recomb"> In vitro recombinase characterization protocol</a></p> | + | |
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p> | + | |
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| - | <h3 class="title3">AND1</h3>
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| - | <h2 class="faq" title="Click to open the FAQ"> In vitro</h2>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/6/6b/XOR1_bio_80.jpg" class="imgcontent" />
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| - | <img style="width:340px; float:right;" src="https://static.igem.org/mediawiki/2013/7/78/Graph.jpg" class="imgcontent" />
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <img style="width:340px; float:left;" src="https://static.igem.org/mediawiki/2013/2/20/XOR1_petit.jpg" class="imgcontent" />
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <p class="texte"><i>Description des conditions d'expérimentation</i></p>
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| - | <p class="texteprotocol"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb">In vitro recombinase characterization protocol</a></p>
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| - | <img style="width:20px" src="https://static.igem.org/mediawiki/2013/2/23/Top_arrow.png"class="imgcontent2" /><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results">Back to the top</a></p>
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| - | <h2 class="faq" title="Click to open the FAQ"> In vivo</h2>
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| - | <p class="texte">The couple TetR-pTet system construction is a first step to characterize the entire system. A constitutive promoter (BBa_J23116) was assemble to tetR and then assemble to pTet-rbs-RFP-term (Bba_I13521). pTet is a regulator constitutively ON that expresses the mRFP1 protein. TetR is an inducer that binds to pTet promoter and thus, represses expression the downstream system. Supply of tetracycline or its analog aTc (anhydrotetracycline) is known to bind to tetR and invert the operation (inhibits expression of mRFP in this case).<br>
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| - | After 18 hours, clones containing this assembly (BBa_J23116-TetR-pTet-RFP) show a leaky basal expression of mRFP. We suppose that the promoter was too weak to express TetR in large quantity. Assembly of a stronger promoter could improve the system and lock the response to an ON/OFF response. <br>
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| - | Besides, an experience was done to analyze the effect of the aTc inducer. Result show a visible induction of the red fluorescent protein expression by addition of aTC (60 ng/mL).</p>
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