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Team:Valencia-CIPF/Strategy
From 2013.igem.org
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| + | <p align="justify"><h2><span style="color: #d64729;"><b>Strategy</b></span></h2> | ||
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| + | The strategy followed was to amplify the genes of the repellent and the aromas from the cDNA obtained from other laboratories by PCR and then clone them into <i>E. coli</i> under the control of different promoters and study its expression. | ||
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| + | Afterwards, do the same in yeast. | ||
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| + | To do this, some of the tools used were: DNA quantification by nanodrop, gel electrophoresis, plasmid extraction, production of competent cells, restriction enzyme digestions and cloning. | ||
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Latest revision as of 00:03, 5 October 2013
Strategy
The strategy followed was to amplify the genes of the repellent and the aromas from the cDNA obtained from other laboratories by PCR and then clone them into E. coli under the control of different promoters and study its expression.
Afterwards, do the same in yeast.
To do this, some of the tools used were: DNA quantification by nanodrop, gel electrophoresis, plasmid extraction, production of competent cells, restriction enzyme digestions and cloning.
Afterwards, do the same in yeast.
To do this, some of the tools used were: DNA quantification by nanodrop, gel electrophoresis, plasmid extraction, production of competent cells, restriction enzyme digestions and cloning.
