Team:Paris Saclay/Notebook/August/20

From 2013.igem.org

(Difference between revisions)
(→1 - Sequences analysis)
 
(10 intermediate revisions not shown)
Line 7: Line 7:
==='''A - Aerobic/Anaerobic regulation system'''===
==='''A - Aerobic/Anaerobic regulation system'''===
-
===='''Objective : obtaining Bba_K1155004, Bba_K1155005, Bba_K1155006'''====
+
===='''Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006'''====
===='''1 - Sequences analysis'''====
===='''1 - Sequences analysis'''====
Line 13: Line 13:
Damir, XiaoJing
Damir, XiaoJing
-
...
+
{|
 +
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
 +
Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.
 +
|}
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''===
Line 19: Line 22:
===='''Objective : obtaining FNR and BphR2 proteins'''====
===='''Objective : obtaining FNR and BphR2 proteins'''====
-
===='''1 - PCR of BphR2 Part I'''====
+
===='''1 - Electrophoresis of PCR products :  BphR2 Part I'''====
-
 
+
-
Damir
+
-
 
+
-
Used quantities :
+
-
* Oligo 54F : 2µL
+
-
* Oligo 55R : 2µL
+
-
* DNA : 1µL
+
-
* Buffer Phusion : 10µL
+
-
* dNTP : 1µL
+
-
* Phusion : 1µL
+
-
* DMS9 : 2µL ??????????????????????????????????
+
-
* H2O : 31µL 
+
-
 
+
-
PCR program :
+
-
 
+
-
[[File:Pstest.jpg|400px]]
+
-
 
+
-
===='''2 - Electrophoresis of PCR products :  BphR2 Part I'''====
+
Nadia
Nadia
{|
{|
-
| style="width:250px;border:1px solid black;" | [[]]
+
| style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]]
| style="width:350px;border:1px solid black;vertical-align:top;" |
| style="width:350px;border:1px solid black;vertical-align:top;" |
*Well 1 : 6µL DNA Ladder
*Well 1 : 6µL DNA Ladder
-
*Well 2 : 40µL BphR2 PartI+8µl of 6X loading dye
+
*Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
*Gel : 0.8%
*Gel : 0.8%
|}  
|}  
 +
Expected sizes :
 +
* BphR2 Part I : 178bp
{|
{|
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" |
-
We obtain a frangment at the right size. We can purify it.
+
We obtained a frangment at the right size. We can purify it.
-
|}  
+
|}
====2 - Gel purification of PCR products : BphR2 Part I ====
====2 - Gel purification of PCR products : BphR2 Part I ====
Line 59: Line 46:
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
-
 
-
Nanodrop :
 
-
* BphR2 Part I: .........
 
  {|
  {|

Latest revision as of 01:30, 5 October 2013

Navigation Home Team Project Labwork Notebook Human practices

Contents

Notebook : August 20

Lab work

A - Aerobic/Anaerobic regulation system

Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006

1 - Sequences analysis

Damir, XiaoJing

Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006.

A - Aerobic/Anaerobic regulation system / B - PCB sensor system

Objective : obtaining FNR and BphR2 proteins

1 - Electrophoresis of PCR products : BphR2 Part I

Nadia

Psgel2008.jpg
  • Well 1 : 6µL DNA Ladder
  • Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye
  • Gel : 0.8%

Expected sizes :

  • BphR2 Part I : 178bp

We obtained a frangment at the right size. We can purify it.

2 - Gel purification of PCR products : BphR2 Part I

Damir, Nadia

Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]

We lost our fragment. We will do the PCR again.


Previous day Back to calendar Next day

Retrieved from "http://2013.igem.org/Team:Paris_Saclay/Notebook/August/20"