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Team:Paris Saclay/Notebook/August/20
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==='''A - Aerobic/Anaerobic regulation system'''=== | ==='''A - Aerobic/Anaerobic regulation system'''=== | ||
| - | ===='''Objective : obtaining | + | ===='''Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006'''==== |
===='''1 - Sequences analysis'''==== | ===='''1 - Sequences analysis'''==== | ||
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | Sequencies were good. We | + | Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
| - | |} | + | |} |
==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensor system'''=== | ||
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| - | | style="width:250px;border:1px solid black;" | [[]] | + | | style="width:250px;border:1px solid black;" | [[File:Psgel2008.jpg|250px]] |
| style="width:350px;border:1px solid black;vertical-align:top;" | | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
*Well 1 : 6µL DNA Ladder | *Well 1 : 6µL DNA Ladder | ||
| - | *Well 2 : 40µL BphR2 | + | *Well 2 : 40µL BphR2 Part I + 8µl of 6X loading dye |
*Gel : 0.8% | *Gel : 0.8% | ||
|} | |} | ||
| + | Expected sizes : | ||
| + | * BphR2 Part I : 178bp | ||
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| style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| - | We | + | We obtained a frangment at the right size. We can purify it. |
| - | |} | + | |} |
====2 - Gel purification of PCR products : BphR2 Part I ==== | ====2 - Gel purification of PCR products : BphR2 Part I ==== | ||
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Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ] | Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ] | ||
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Latest revision as of 01:30, 5 October 2013
Contents |
Notebook : August 20
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining BBa_K1155004, BBa_K1155005, BBa_K1155006
1 - Sequences analysis
Damir, XiaoJing
|
Sequencies were good. We obtained : BBa_K1155004, BBa_K1155005, BBa_K1155006. |
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : obtaining FNR and BphR2 proteins
1 - Electrophoresis of PCR products : BphR2 Part I
Nadia
|
|
Expected sizes :
- BphR2 Part I : 178bp
|
We obtained a frangment at the right size. We can purify it. |
2 - Gel purification of PCR products : BphR2 Part I
Damir, Nadia
Protocol : [http://www.mn-net.com/tabid/1452/default.aspx Gel purification ]
|
We lost our fragment. We will do the PCR again. |
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