Team:INSA Toulouse/contenu/extras/achievements

From 2013.igem.org

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   <h2 class="title2">Foreword</h2>
   <h2 class="title2">Foreword</h2>
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   <p class="texte">For this project, our goal is and always has been the bacterial calculator. Because this project is beautiful, clever and ambitious.<br>
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<div class="list">
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However, we knew it was impossible to complete in time. However, we could show that the project was feasible. For this, we focused on the characterization of the different parts of the project separately.<br>
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Showing that each part works individually, we have collected good arguements to support the feasibility of the project.</p>
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      <li>Our initial project was the full realization of the <i>E. calculus</i> strain. We even thought of selling the idea to TI <img src="https://static.igem.org/mediawiki/2013/d/db/Smiley_10241.jpg"/>. However, we soon realized that the full realization would probably take (a little <img src="https://static.igem.org/mediawiki/2013/d/db/Smiley_10241.jpg"/>) longer than the three months devoted to the iGEM competition. Furthermore, we are also aware that integration of many independant biological modules is always more complicated than it seems. However, a first step toward integration is the demonstration that each biological module works individually as expected. We would then have good arguments in support of the <span class="textebold">feasibility</span> of the whole project.
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      </li>
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  <br>
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      <li>Our secondary goal was therefore to demonstrate the validity of our modules design. We then reorientated our experiments toward the characterisation of the different modules. Each module thus can be used independently by other iGEM teams because the submitted parts were designed without the output genes. Most intermediate molecular biology constructions are also deposited in the registry to facilitate the redesign of the modules. The results we have obtained are very promising and we hope that the modules we have constructed and characterized will have a long life in other worldwide iGEM teams, way after our 2013 project.  
 +
      </li>
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  <br>
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      <li>Interdisciplinarity is a pillar in Synthetic Biology. iGEM projects aim at modeling the biological reactions that were designed to promote a full integration of wet and dry lab experiments. Modeling the entire <i>E. calculus</i> system did not seem either feasible or even conceivable. We focussed on only part of the project. In our whole <i> E. calculus</i> design, the diffusion of the carry was critical to implement the bacterial full adder. We then considered that modelling the diffusion of the carry molecule (AHL) was essential to fully support the feasibility of the design. Our results will provide interesting concepts that we hope will serve for similar design where diffusion of small molecules are necessary to achieve specific biological functions.
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      </li>
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  <p class="texte">A faire : Expliquer le projet = calculateur on a a choisit de le décomposer en modules et de les caractériser un à un. Ainsi chaque module peut etre utilisé indépendamment dans d'autres projects. voici comment on été pensé chaque module, courte phrase avec lien vers le spages biological modules puis le projet en global lien vers la page e.calculus design et enfin voici ce qu'on a réussi à faire lien vers la page results. On s'est aussi intérressé a la partie éthique on a eu des réfléxions ethiques sur la bio de synthèse sur igem et sur le project. Voici où on ets est actuellement et pourquoi on vise une médaille d'or avec le tableau récapitulatif...</p>
 
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   <h2 class="title2">Medals fulfillment</h2>   
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   <h2 class="title2">Accomplishment</h2>   
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<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;"><center>Bronze Medal </center><img src="https://static.igem.org/mediawiki/2013/8/86/Medal_bronze.png" class="imgcontenttable"/></td>
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<td style="border-bottom:4px solid #e5e6e6; border-top-left-radius:9px;"><center><br>Bronze Medal <img src="https://static.igem.org/mediawiki/2013/8/86/Medal_bronze.png" class="imgcontenttable"/></center><br></td>
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<td style="border-bottom:4px solid #e5e6e6;"><center>Silver Medal  </center><img src="https://static.igem.org/mediawiki/2013/d/dd/Medal_silver.png" class="imgcontenttable"/></td>
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<td style="border-bottom:4px solid #e5e6e6;"><center><br>Silver Medal  <img src="https://static.igem.org/mediawiki/2013/d/dd/Medal_silver.png" class="imgcontenttable"/></center><br></td>
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<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;"><center>Gold Medal</center><img src="https://static.igem.org/mediawiki/2013/b/bd/Medal_gold_3.png" class="imgcontenttable"/></td>
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<td style="border-bottom:4px solid #e5e6e6; border-top-right-radius:9px;"><center><br>Gold Medal<img src="https://static.igem.org/mediawiki/2013/b/bd/Medal_gold_3.png" class="imgcontenttable"/></center><br></td>
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<td style="border-right:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/>1. Team registration</td>
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<td style="border-right:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/>Team registration</td>
<td style="border-right:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.</td>
<td style="border-right:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/>Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.</td>
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<td><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry </td>
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<td><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry : <a href="http://parts.igem.org/Part:BBa_K1132031"target="_blank">BBa_K1132031</a>, <a href="http://parts.igem.org/Part:BBa_K1132032"target="_blank">BBa_K1132032</a>, <a href="http://parts.igem.org/Part:BBa_K1132038"target="_blank">BBa_K1132038</a> </td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Complete Judging form </td>
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Complete Judging form </td>
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/>Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/>Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry
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Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)</td>
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Submit this new part to the iGEM Parts Registry <a href="http://parts.igem.org/Part:BBa_K1132043"target="_blank">BBa_K1132043</a></td>
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<td style="border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system</td>
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<td style="border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system →<a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/team/partners/iGEM_buddies" target="_blank"> iGEM Buddies </a> </td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Team Wiki </td>
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Team Wiki </td>
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)</td>
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines)</td>
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<td style="border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe a novel approach that your team has used to help you and others consider these aspects of the design and outcomes of synthetic biology efforts.</td>
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<td style="border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe a novel approach that your team has used to help you and others consider these aspects of the design and outcomes of synthetic biology efforts. → <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/novel_approach" target="_blank">Novel Approach</a> </td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Present a poster and a talk at the iGEM Jamboree </td>
<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Present a poster and a talk at the iGEM Jamboree </td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project</td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project. → <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/human_practice/ethical_aspects" target="_blank"> Ethical Aspect Page</a></td>
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<td style="border-top:1px solid #e5e6e6;"></td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry </td>
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<td style="border-right:1px solid #e5e6e6; border-top:1px solid #e5e6e6;"><img style="width:20px;" src="https://static.igem.org/mediawiki/2013/c/c1/Icone-check-pink.png" class="imgcontenttableleft"/> Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry : <a href="http://parts.igem.org/Part:BBa_K1132001" target="_blank">BBa_K1132001</a>, <a href="http://parts.igem.org/Part:BBa_K1132002"target="_blank">BBa_K1132002</a>, <a href="http://parts.igem.org/Part:BBa_K1132003"target="_blank">BBa_K1132003</a>, <a href="http://parts.igem.org/Part:BBa_K1132004"target="_blank">BBa_K1132004</a>. </td>
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<p class="texte"> - <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb" target="_blank">Best BioBrick Measurement Approach</a><br>
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- <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/results/carry" target="_blank">Best Model</a></p>
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<p class="texte"> → <a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/extras/attribution" target="_blank">Attribution Page</a></p>
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Latest revision as of 03:42, 5 October 2013

logo


Achievements

Foreword

  • Our initial project was the full realization of the E. calculus strain. We even thought of selling the idea to TI . However, we soon realized that the full realization would probably take (a little ) longer than the three months devoted to the iGEM competition. Furthermore, we are also aware that integration of many independant biological modules is always more complicated than it seems. However, a first step toward integration is the demonstration that each biological module works individually as expected. We would then have good arguments in support of the feasibility of the whole project.

  • Our secondary goal was therefore to demonstrate the validity of our modules design. We then reorientated our experiments toward the characterisation of the different modules. Each module thus can be used independently by other iGEM teams because the submitted parts were designed without the output genes. Most intermediate molecular biology constructions are also deposited in the registry to facilitate the redesign of the modules. The results we have obtained are very promising and we hope that the modules we have constructed and characterized will have a long life in other worldwide iGEM teams, way after our 2013 project.

  • Interdisciplinarity is a pillar in Synthetic Biology. iGEM projects aim at modeling the biological reactions that were designed to promote a full integration of wet and dry lab experiments. Modeling the entire E. calculus system did not seem either feasible or even conceivable. We focussed on only part of the project. In our whole E. calculus design, the diffusion of the carry was critical to implement the bacterial full adder. We then considered that modelling the diffusion of the carry molecule (AHL) was essential to fully support the feasibility of the design. Our results will provide interesting concepts that we hope will serve for similar design where diffusion of small molecules are necessary to achieve specific biological functions.

Medals fulfillment


Bronze Medal


Silver Medal


Gold Medal

Team registration Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. Improve the function of an existing BioBrick Part or Device (created by another team or your own institution in a previous year), enter this information in the Registry : BBa_K1132031, BBa_K1132032, BBa_K1132038
Complete Judging form Document the characterization of this part in the 'Main Page' of that Part's/Device's Registry entry Submit this new part to the iGEM Parts Registry BBa_K1132043 Help any registered iGEM team from another school or institution by, for example, characterizing a part, debugging a construct, or modeling or simulating their system → iGEM Buddies
Team Wiki Submit this new part to the iGEM Parts Registry (submissions must adhere to the iGEM Registry guidelines) Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe a novel approach that your team has used to help you and others consider these aspects of the design and outcomes of synthetic biology efforts. → Novel Approach
Present a poster and a talk at the iGEM Jamboree Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing. Describe one or more ways in which these or other broader implications have been taken into consideration in the design and execution of your project. → Ethical Aspect Page
Document at least one new standard BioBrick Part or Device used in your project/central to your project and submit this part to the iGEM Registry : BBa_K1132001, BBa_K1132002, BBa_K1132003, BBa_K1132004.

Special Prices

- Best BioBrick Measurement Approach
- Best Model

Attribution and Contributions

Attribution Page