Sensor Model

Introduction

In order to determine our iron-coli's enterobactin production rate, we first have to know how much time our bacteria will take to sense the ambient iron. So, this first part of the Enterobactin production model focuses on the synthetic sensing system our team implemented in the bacteria.

Observations

As shown on the Figure 1, our sensing system relies on a FUR Binding Site (FBS). In order to easily model the sensing delay and analyse its results, the first construction is composed of a GFP placed right after the FBS.

Figure 1:

First construction

Goals

Our goal in this part of the model is to create a generic FBS-related sensing model so that:

• We can determine the iron-sensing delay of our bacteria
• The model can can be reused by other iron related projects

Materials and methods

Results

Conclusion

Models and scripts