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Team:UC Davis/Parts
From 2013.igem.org
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<td> Amy Soon</td> | <td> Amy Soon</td> | ||
<td> 1003</td> | <td> 1003</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr class="lightColor"> | ||
| + | <td><a target="new" href="http://parts.igem.org/Part:BBa_K1212021"> BBa_K1212021</a></td> | ||
| + | <td> Composite</td> | ||
| + | <td> J23100+TBS 2+RBS+GFP</td> | ||
| + | <td> Amy Soon</td> | ||
| + | <td> 988</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr class="darkColor"> | ||
| + | <td><a target="new" href="http://parts.igem.org/Part:BBa_K1212022"> BBa_K1212022</a></td> | ||
| + | <td> Composite</td> | ||
| + | <td> J23101+TBS 2+RBS+GFP</td> | ||
| + | <td> Amy Soon</td> | ||
| + | <td> 974</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr class="lightColor"> | ||
| + | <td><a target="new" href="http://parts.igem.org/Part:BBa_K1212023"> BBa_K1212023</a></td> | ||
| + | <td> Composite</td> | ||
| + | <td> J23105+TBS 2+RBS+GFP</td> | ||
| + | <td> Amy Soon</td> | ||
| + | <td> 974</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr class="darkColor"> | ||
| + | <td><a target="new" href="http://parts.igem.org/Part:BBa_K1212024"> BBa_K1212024</a></td> | ||
| + | <td> Composite</td> | ||
| + | <td> J23106+TBS 2+RBS+GFP</td> | ||
| + | <td> Amy Soon</td> | ||
| + | <td> 988</td> | ||
| + | </tr> | ||
| + | |||
| + | <tr class="lightColor"> | ||
| + | <td><a target="new" href="http://parts.igem.org/Part:BBa_K1212025"> BBa_K1212025</a></td> | ||
| + | <td> Composite</td> | ||
| + | <td> J23109+TBS 2+RBS+GFP</td> | ||
| + | <td> Amy Soon</td> | ||
| + | <td> 974</td> | ||
</tr> | </tr> | ||
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<div class="floatboxwide"> | <div class="floatboxwide"> | ||
<p> | <p> | ||
| - | Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization. | + | Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization. The pBAD+Riboswitch+GFP parts (BBa_K1212009 and BBa_K1212010) were originally planned for testing our riboswitches in case the RiboTAL constructs were not working. Since the RiboTALs did work as expected, we focused on characterizing the RiboTALs over the riboswitch testing constructs. |
</p> | </p> | ||
</div> | </div> | ||
| + | <!--Begin Showbox--> | ||
<div class="floatboxwide"> | <div class="floatboxwide"> | ||
<table class="showbox"> | <table class="showbox"> | ||
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<td> | <td> | ||
| - | <a href="https://2013.igem.org/Team:UC_Davis/ | + | <div><a href="https://2013.igem.org/Team:UC_Davis/Project_Overview"> |
| - | <img src="https://static.igem.org/mediawiki/2013/b/bf/TALpic_UCDavis.jpg" class="blur"> | + | <img src="https://static.igem.org/mediawiki/2013/b/bf/TALpic_UCDavis.jpg" class="blur"></a> |
| - | </ | + | </div> |
| - | <a href="https://2013.igem.org/Team:UC_Davis/ | + | <a href="https://2013.igem.org/Team:UC_Davis/Project_Overview"><h3>Project Overview</h3></a> |
<p>Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression. | <p>Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression. | ||
</p></a> | </p></a> | ||
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<a href="https://2013.igem.org/Team:UC_Davis/Data"><img src="https://static.igem.org/mediawiki/2013/d/d5/Resultsicon_UCDavis.jpg" class="blur"></a> | <a href="https://2013.igem.org/Team:UC_Davis/Data"><img src="https://static.igem.org/mediawiki/2013/d/d5/Resultsicon_UCDavis.jpg" class="blur"></a> | ||
<a href="https://2013.igem.org/Team:UC_Davis/Data"><h3>Results</h3></a> | <a href="https://2013.igem.org/Team:UC_Davis/Data"><h3>Results</h3></a> | ||
| - | <p>Check out the results of our experiments. | + | <p>Check out the cool results of our experiments with RiboTALs. |
</p> | </p> | ||
</td> | </td> | ||
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<tr> | <tr> | ||
<td> | <td> | ||
| - | <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><img src="https://static.igem.org/mediawiki/igem.org/ | + | <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"> |
| + | <img src="https://static.igem.org/mediawiki/2013/3/35/Humanpracbutton2_UCDavis.jpg" class="blur" /> | ||
| + | <!--img src="https://static.igem.org/mediawiki/2013/9/97/UCD_2013_HO_Button.jpg" class="blur"--></a> | ||
<a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><h3>Human Practices</h3></a> | <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><h3>Human Practices</h3></a> | ||
| - | <p>Take a look at how we | + | <p>Take a look at how we promote sharing in iGEM through The Depot, an open BioBrick characterization database.<br /> |
| + | <a href="http://dilbert.cs.ucdavis.edu/Depot" class="bold">Visit the Depot!</a> | ||
</p> | </p> | ||
</td> | </td> | ||
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</div> | </div> | ||
| + | <!--End Showbox--> | ||
| + | |||
<div id="sitemapbox" class="floatboxwide"></div> | <div id="sitemapbox" class="floatboxwide"></div> | ||
Latest revision as of 06:12, 27 October 2013
Parts
The following parts were assembled throughout the development of this project:| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1212011 | Composite | pBAD+Riboswitch 2+TAL1 | Amy Soon | 2612 |
| BBa_K1212012 | Composite | pBAD+Riboswitch 2+TAL8 | Amy Soon | 2616 |
| BBa_K1212013 | Composite | pTet+TBS 1+RBS+GFP+T | Aura Ferreiro | 1003 |
| BBa_K1212014 | Composite | pBAD+Riboswitch 1+TAL 1 | Amy Soon | 2616 |
| BBa_K1212015 | Composite | pBAD+Riboswitch 1+TAL 8 | Amy Soon | 2620 |
| BBa_K1212016 | Composite | pTet+TBS 2+RBS+GFP | Amy Soon | 1003 |
| BBa_K1212021 | Composite | J23100+TBS 2+RBS+GFP | Amy Soon | 988 |
| BBa_K1212022 | Composite | J23101+TBS 2+RBS+GFP | Amy Soon | 974 |
| BBa_K1212023 | Composite | J23105+TBS 2+RBS+GFP | Amy Soon | 974 |
| BBa_K1212024 | Composite | J23106+TBS 2+RBS+GFP | Amy Soon | 988 |
| BBa_K1212025 | Composite | J23109+TBS 2+RBS+GFP | Amy Soon | 974 |
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K1212002 | Plasmid_Backbone | Golden Gate Compatible pSB3K3 | Alex Neckelmann | 2750 |
| BBa_K1212006 | Composite | pBAD+Golden Gate Compatible GFP | Aura Ferreiro | 1052 |
| BBa_K1212009 | Composite | pBAD+Riboswitch 2+GFP+T | Amy Soon | 1086 |
| BBa_K1212010 | Composite | pBAD+Riboswitch 1+GFP+T | Amy Soon | 1090 |
Our best characterized parts are our RiboTAL constructs, which consist of parts BBa_K1212011-BBa_K1212016. Among these parts, most of characterization was focused on the riboswitch+TAL constructs (BBa_K1212011, BBa_K1212012, BBa_K1212014, BBa_K1212015), as the other parts were simply pTet+GFP, which are already well characterized parts, with some extra base pairs added for our TAL binding sites (BBa_K1212013, BBa_K1212016). Both our pSB3K3 backbone (BBa_K1212002) and our GFP (BBa_K1212006) with their BsaI sites fixed were used in our RiboTAL constructs and functioned as expected but did not need separate characterization. The pBAD+Riboswitch+GFP parts (BBa_K1212009 and BBa_K1212010) were originally planned for testing our riboswitches in case the RiboTAL constructs were not working. Since the RiboTALs did work as expected, we focused on characterizing the RiboTALs over the riboswitch testing constructs.
Project OverviewLearn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression. |
ResultsCheck out the cool results of our experiments with RiboTALs. |
Human PracticesTake a look at how we promote sharing in iGEM through The Depot, an open BioBrick characterization database. |
Judging CriteriaHere's the criteria that we met for this year's team. |
