Team:USTC CHINA/Parts

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<body background="https://static.igem.org/mediawiki/2013/6/62/2013ustc-china_Light_grey_bg.png">
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<div class="bar" align="center">
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    <div class="container" align="left">
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<div id="igemlogo"><a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a></div>
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<ul id="nav">
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
 +
        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
 +
            <ul class="subs">
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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            </ul>
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        </li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
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              <ul class="subs">
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
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              </ul>
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        </li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
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              <ul class="subs">
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
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              </ul>
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        </li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
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              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
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              </ul>
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        </li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
 +
              <ul class="subs">
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
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                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
 +
                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
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                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
 +
              </ul>
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        </li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
 +
    </ul>
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        <div id="tlogo"><img src="https://static.igem.org/mediawiki/2013/f/f8/2013ustc-china_T-VACCINE.png" width="100%" height="123" />
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<div class="conbar1">
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<div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a> &gt; <a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div></div>
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<div class="part-col-1">
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        <h1>TD1, Transdermal peptide</h1>
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         <a href="https://igem.org/Team_Wikis?year=2013"><img id="iGEM_logo"  
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         <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074000">BBa_K1074000</a></h2>
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        <p align="center">TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...</p>
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</div>
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src="https://static.igem.org/mediawiki/igem.org/4/48/Peking_igemlogo.jpg"/></a>
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<div class="part-col-1">
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        <h1>Pgrac+RBS+SamyQ+TD1<br>+GFP</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074006">BBa_K1074006</a></h2>
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        <p align="center">This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...</p>
</div>
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        <h1>PHT43</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074001">BBa_K1074001</a></h2>
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    <div id="ModelOverviewContainer">
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        <p align="center">PHT43 is a <i>E.coli-B.subtilis</i> shuttle vector allowing high-level expression of secreted proteins in <i>B.subtilis</i>... </p>
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          <img id="ModelOverviewBackground"  
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src="https://static.igem.org/mediawiki/igem.org/a/a4/Peking2013_Datapage_background.jpg" />
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<div class="part-col-2">
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          <h1 id="ModelOverviewTitle">Parts</h1>
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        <h1>HBsAg</h1>
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          <h1 id="MoedlOverviewIntroduction"></h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074002">BBa_K1074002</a></h2>  
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          <p id="ModelOverviewContent"><br/>
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        <p align="center">HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...</p>
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        <h1>Ag85b</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074003">BBa_K1074003</a></h2>  
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        <p align="center">The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin... </p>
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text-align:justify; line-height:25px;"><b>TD1, Transdermal peptide</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1074000">Main Page-BBa_K1074000</a>
+
-
</br>TD1 is a short synthetic peptide(ACSSSPSKHCG)identified by in vivo phage
+
-
 
+
-
display, facilitated efficient transdermal protein delivery through intact
+
-
 
+
-
skin. Studies suggested that the peptide creates a transient opening in the
+
-
 
+
-
skin barrier to enable macromolecular material to reach systemic circulation
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
    <div id="FavoritePart2" class="SmallBoxs">
+
-
<p id="Favoritecontent2" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px;font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>Pgrac+RBS+SamyQ+TD1+GFP</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1074006">Main Page-BBa_K1074006</a>
+
-
This is the main circuit of our project to allow high expression of target
+
-
 
+
-
protein(antigen,adjuvant).GFP can be substituted by various proteins via a
+
-
 
+
-
modular PCR or standard cut/ligation method.
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
    <div id="FavoritePart3" class="SmallBoxs">
+
-
<p id="Favoritecontent3" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px; font-size:18px;font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>PHT43</b></br>
+
-
PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of
+
-
 
+
-
secreted proteins in B.subtilis. It is based on the strong σA-dependent
+
-
 
+
-
promoter preceding the groE operon of B. subtilis which has been converted into
+
-
 
+
-
an efficiently controllable (IPTGinducible)promoter by addition of the lac
+
-
 
+
-
operator. An efficient Shine-Dalgarno (SD) sequence as well as a multiple
+
-
 
+
-
cloning site (BamH I, Xba I, Aat II, Sma I) were also inserted. To obtain
+
-
 
+
-
secretion of recombinant proteins, the coding region for the signal peptide of
+
-
 
+
-
the amyQ gene encoding an α-amylase was fused to the SD sequence.
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
    <div id="FavoritePart4" class="SmallBoxs">
+
-
<p id="Favoritecontent4" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px; font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>HBsAg</b></br>
+
-
HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates
+
-
 
+
-
current hepatitis B infection.
+
-
Sequence and Features
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
 
+
-
/*
+
-
<div id="FavoritePart5" class="SmallBoxs">
+
-
<p id="Favoritecontent5" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px; font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>Ag85b</b></br>
+
-
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high  
+
-
 
+
-
affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which
+
-
 
+
-
facilitates the attachment of M.tuberculosis to murine alveolar macrophages
+
-
 
+
-
(AMs). They also help to maintain the integrity of the cell wall by catalyzing
+
-
 
+
-
the transfer of mycolic acids to cell wall arabinogalactan and through the
+
-
 
+
-
synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze
+
-
 
+
-
the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose
+
-
 
+
-
monomycolate (TMM) to another TMM, leading to the formation of TDM.
+
-
Sequence and Features
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
 
+
-
 
+
-
 
+
-
<div id="FavoritePart6" class="SmallBoxs">
+
-
<p id="Favoritecontent6" style="position:absolute; top:20px; width:360px;
+
-
 
+
-
left:20px; font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>
+
-
Protective Antigen Domain 4</b></br>
+
-
Protective antigen (PA) is the central component of the three-part protein
+
-
 
+
-
toxin secreted by Bacillus anthracis, the organism responsible for anthrax.
+
-
 
+
-
Homologues of PA have been found in several spore-forming Gram-positive
+
-
 
+
-
bacteria, and share the ability to translocate toxic enzymes into the host
+
-
 
+
-
cytosol.The PA monomer is organised mainly into antiparallel beta-sheets and
+
-
 
+
-
has four domains: an N-terminal domain (domain 1) containing two calcium ions
+
-
 
+
-
and the cleavage site for activating proteases; a heptamerisation domain
+
-
 
+
-
(domain 2) containing a large flexible loop implicated in membrane insertion; a
+
-
 
+
-
small domain of unknown function (domain 3); and a carboxy-terminal receptor-
+
-
 
+
-
binding domain (domain 4) [PMID: 9039918].
+
-
This entry represents domain 4 of PA, which has an immunoglobulin-like fold.
+
-
 
+
-
Domain 4 plays a key role in cellular receptor recognition, as well as in pH-
+
-
 
+
-
dependent pore formation[PMID: 19722284].
+
-
</br>
+
-
 
+
-
</p>
+
-
    </div>
+
-
 
+
-
 
+
-
*/
+
-
 
+
-
    <div id="ImprovedPart">
+
-
<p id="Favoritecontent1" style="position:absolute; top:20px; right:20px;
+
-
 
+
-
left:20px; font-size:18px; font-family:calibri,Arial, Helvetica, sans-serif;
+
-
 
+
-
text-align:justify; line-height:25px;"><b>NahR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031610">Main Page-
+
-
 
+
-
BBa_K1031610</a></br>
+
-
Pre-existing Part: <a href="http://partsregistry.org/Part:BBa_J61051">Main
+
-
 
+
-
Page-BBa_J61051</a>
+
-
</br>
+
-
BBa_K1031610 is upgraded from previous part BBa_J61051 designed by Arkin Lab.
+
-
NahR is a 34 kDa transcription factor mined from <i>Pseudomonas putida</i>
+
-
 
+
-
which binds to <i>nah</i> and <i>sal</i> promoters to activate transcription in
+
-
 
+
-
response to the inducer salicylate. We constructed a Psal/NahR biosensor
+
-
 
+
-
circuit using sfGFP as reporter gene. Adaptor of this biosensor not only
+
-
 
+
-
improves detection efficiency but also expands detection profile.
+
-
</br>
+
-
<a href="https://2013.igem.org/Team:Peking/Project/BioSensors/NahR">Visit
+
-
 
+
-
Wiki</a>
+
-
</p>
+
-
    </div>
+
-
 
+
-
<div id="OtherPart">
+
-
<p id="Summary1" style="position:relative; left:20px; font-size: 25px; font-
+
-
 
+
-
family:calibri,Arial, Helvetica, sans-serif;line-height:40px;" >
+
-
<b>Parts for Biosensors</b>
+
-
</p>
+
-
<p style="position: relative; top:0px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>DmpR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031221">BBa_K1031221</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031222">BBa_K1031222</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031223">BBa_K1031223</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031224">BBa_K1031224</a> are the
+
-
 
+
-
RBS library of DmpR biosensor's reporter. </br>
+
-
</p>
+
-
<p style="position: relative; top:20px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>HbpR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031300">BBa_K1031300</a> is the
+
-
 
+
-
coding sequence of HbpR with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031301">BBa_K1031301</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031302">BBa_K1031302</a> are the
+
-
 
+
-
RBS library of HbpR biosensor's reporter. </br>
+
-
</p>
+
-
<p style="position: relative; top:40px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>HcaR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031410">BBa_K1031410</a> is the
+
-
 
+
-
coding sequence of HcaR with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031441">BBa_K1031441</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031442">BBa_K1031442</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031443">BBa_K1031443</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031444">BBa_K1031444</a> are the
+
-
 
+
-
Pc library of HbpR to tune its performance . </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031420">BBa_K1031420</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031421">BBa_K1031421</a> are the
+
-
 
+
-
RBS library of HcaR biosensor's reporter. </br>
+
-
 
+
-
</p>
+
-
<p style="position: relative; top:50px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>CapR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031100">BBa_K1031100</a> is the
+
-
 
+
-
coding sequence of CapR transcriptional factor. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031111">BBa_K1031111</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031112">BBa_K1031112</a> are basic
+
-
 
+
-
sensor construction with different CapR expression level.  </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031113">BBa_K1031113</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031114">BBa_K1031114</a> are
+
-
 
+
-
reporters with different RBS for the CapR biosensor. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:60px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>HpaR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031510">BBa_K1031510</a> is the
+
-
 
+
-
coding sequence of HpaR transcriptional factor. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031520">BBa_K1031520</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031521">BBa_K1031521</a> are
+
-
 
+
-
reporters with different RBS for the HpaR biosensor. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:70px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>NahR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031610">BBa_K1031610</a> is the
+
-
 
+
-
NahR biosensor circuit.</br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:80px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>PaaX</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031710">BBa_K1031710</a> is the
+
-
 
+
-
coding sequence of PaaX with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031742">BBa_K1031742</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031743">BBa_K1031743</a> and
+
-
<a href="http://partsregistry.org/Part:BBa_K1031744">BBa_K1031744</a> are the
+
-
 
+
-
Pc library of PaaX. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:90px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>XylS</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031911">BBa_K1031911</a> is the
+
-
 
+
-
coding sequence of XylS transcriptional factor with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031912">BBa_K1031912</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031913">BBa_K1031913</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031916">BBa_K1031916</a> are the
+
-
 
+
-
Pc library of XylS. </br>
+
-
</p>
+
-
 
+
-
<p style="position: relative; top:100px; left: 20px; width:800px;  font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>XylR</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031803">BBa_K1031803</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031804">BBa_K1031804</a> are the
+
-
 
+
-
RBS library of XylR biosensor's reporter. </br>
+
-
</p>
+
-
 
+
-
 
+
-
<p id="Summary2" style="position:relative; top:120px; left:20px; font-size:
+
-
 
+
-
25px; font-family:calibri,Arial, Helvetica, sans-serif;line-height:40px;" >
+
-
<b>Parts for Adaptors</b>
+
-
 
+
-
<p style="position: relative; top:110px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"><b>NahF</b></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031620">BBa_K1031620</a> is the
+
-
 
+
-
coding sequence of XylS transcriptional factor with terminators. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031621">BBa_K1031621</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031622">BBa_K1031622</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031623">BBa_K1031623</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031624">BBa_K1031624</a> and     
+
-
 
+
-
   
+
-
<a href="http://partsregistry.org/Part:BBa_K1031625">BBa_K1031625</a>are the
+
-
 
+
-
Pc library of NahF. </br>
+
-
</p>
+
-
 
+
-
<p id="Summary3" style="position:relative; top:120px; left:20px; font-size:
+
-
 
+
-
25px; font-family:calibri,Arial, Helvetica, sans-serif;line-height:40px;" >
+
-
<b>Parts for Band-Pass Filter</b>
+
-
<p style="position: relative; top:90px; left: 20px; width:800px; font-
+
-
 
+
-
size:18px;font-family:calibri,Arial, Helvetica, sans-serif; text-align:justify;
+
-
 
+
-
line-height:30px;"></br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031021">BBa_K1031021</a> is the
+
-
 
+
-
hybrid promoter for the reporter node, which can be activated as well as
+
-
 
+
-
inhibited. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031011">BBa_K1031011</a> is the
+
-
 
+
-
transcriptional factor that can activate its cognate promoter, which is used as
+
-
 
+
-
the activate node in the band-pass filter. </br>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031013">BBa_K1031013</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031014">BBa_K1031014</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031015">BBa_K1031015</a>
+
-
<a href="http://partsregistry.org/Part:BBa_K1031016">BBa_K1031016</a> are RBS
+
-
 
+
-
library to tune the expression of C1, under the control of pSal promoter, which
+
-
 
+
-
is a crucial part for band-pass filter. </br>
+
 +
<div class="part-col-2">
 +
        <h1>Protective Antigen Domain 4</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074004">BBa_K1074004</a></h2>
 +
        <p align="center">Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax... </p>
</div>
</div>
-
 
+
<div class="part-col-2">
-
 
+
        <h1>LTB</h1>
-
<div id="Title1">
+
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074005">BBa_K1074005</a></h2>  
-
<p>Favorite Parts</p>
+
        <p align="center">This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of <i>E.coli</i>...</p>
</div>
</div>
-
<div id="Title2">
+
<div class="part-col-2">
-
<p>Improved Parts</p>
+
        <h1>Pctc+RBS+<br>amilCP</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074007">BBa_K1074007</a></h2>
 +
        <p align="center">Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in <i>B.subtilis</i> activated by endogenous sigma factor B ...</p>
</div>
</div>
-
<div id="Title3">
+
<div class="part-col-2">
-
<p>Part List</p>
+
        <h1>SdpA,SdpB,<br>SpbC</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074009">BBa_K1074009</a></h2>
 +
        <p align="center">Killing factor of <i>B.subtilis</i> ,SpbC Induces the lysis of other <i>B.subtilis</i> cells that have not entered the sporulation pathway...</p>
</div>
</div>
 +
<div class="part-col-2">
 +
        <h1>Pgrac+RBS+SDP</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074010">BBa_K1074010</a></h2>
 +
        <p align="center">Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of <i>B.subtilis</i>...</p>
 +
</div>
 +
<div class="clear"></div>
 +
<div class="part-col-2">
 +
        <h1>PsdpRI+RBS<br>+SDP</h1>
 +
      <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074011">BBa_K1074011</a></h2>
 +
        <p align="center">We construct this gene circuit as our kill switch to kill the engineered <i>B.subtilis</i> for the safety purpose...</p>
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<div class="part-col-2">
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        <h1>Pgrac promoter</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074012">BBa_K1074012</a></h2>
 +
        <p align="center">Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG... </p>
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        <h1>promoter sdpR/I</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074013">BBa_K1074013</a></h2>
 +
        <p align="center">This part is a sigma factor A-dependent promotor of the gene sdpR derived from the <i>B.subtilis</i>...</p>
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        <h1>SamyQ</h1>
 +
        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074014">BBa_K1074014</a></h2>
 +
        <p align="center">SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in <i>B.subtilis</i> WB800N...</p>
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        <h1>RBS for Pgrac</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074015">BBa_K1074015</a></h2>
 +
        <p align="center">An efficient RBS for promoter Pgrac(BBa_K1074012) in <i>B.subtilis</i>.../p>
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        <h1>RBS for Pctc</h1>
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        <h2><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1074016">BBa_K1074016</a></h2>
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        <p align="center">This is an efficient RBS for promoter Pctc(BBa_K143010) in <i>B.subtilis</i>...</p>
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<div class="rightbar">
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<div class="port-sidebar-border"><h>Project</h></div>
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<div class="clear"></div>
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<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></div>
 +
<div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Basic Experiment</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/AdvancingWork">Advancing Work</a></div>
 +
<div id="t2"><a href="https://2013.igem.org/Team:USTC_CHINA/Project/FutureWork">Future Work</a></div>
 +
<div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></div>
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</div></div></div>       
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Latest revision as of 12:33, 28 October 2013

TD1, Transdermal peptide

BBa_K1074000

TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...

Pgrac+RBS+SamyQ+TD1
+GFP

BBa_K1074006

This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...

PHT43

BBa_K1074001

PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...

HBsAg

BBa_K1074002

HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...

Ag85b

BBa_K1074003

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...

Protective Antigen Domain 4

BBa_K1074004

Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...

LTB

BBa_K1074005

This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...

Pctc+RBS+
amilCP

BBa_K1074007

Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B.subtilis activated by endogenous sigma factor B ...

SdpA,SdpB,
SpbC

BBa_K1074009

Killing factor of B.subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...

Pgrac+RBS+SDP

BBa_K1074010

Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of B.subtilis...

PsdpRI+RBS
+SDP

BBa_K1074011

We construct this gene circuit as our kill switch to kill the engineered B.subtilis for the safety purpose...

Pgrac promoter

BBa_K1074012

Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...

promoter sdpR/I

BBa_K1074013

This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...

SamyQ

BBa_K1074014

SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in B.subtilis WB800N...

RBS for Pgrac

BBa_K1074015

An efficient RBS for promoter Pgrac(BBa_K1074012) in B.subtilis.../p>

RBS for Pctc

BBa_K1074016

This is an efficient RBS for promoter Pctc(BBa_K143010) in B.subtilis...

Project

Retrieved from "http://2013.igem.org/Team:USTC_CHINA/Parts"