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Team:USTC CHINA/Notebook/Protocols
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| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li> | |
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| - | + | <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li> | |
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| - | <div id="breadcrumb"><a href="">Home</a> > <a href=""> | + | <div id="breadcrumb"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a> > <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>> <a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></div></div> |
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<h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction">Gel Extraction</a></h1> | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Gel Extraction">Gel Extraction</a></h1> | ||
<h1>DNA digestion</h1> | <h1>DNA digestion</h1> | ||
| - | <h2>Single Digestion</h2> | + | <h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Single Digestion">Single Digestion</a></h2> |
| - | <h2>Double Digestion</h2> | + | <h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Double Digestion">Double Digestion</a></h2> |
| - | <h1>Colony PCR</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Colony PCR">Colony PCR</a></h1> |
| - | <h1>PCR</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/PCR">PCR</a></h1> |
| - | <h1>Ligation</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Ligation">Ligation</a></h1> |
| - | <h1>Plasmid mini-prep</h1></div> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Plasmid mini-prep">Plasmid mini-prep</a></h1></div> |
<div class="protocol-col-sub"> | <div class="protocol-col-sub"> | ||
<h1>Transformation</h1> | <h1>Transformation</h1> | ||
| - | <h2.The transformation of E.coli</h2> | + | <h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/The transformation of E.coli">The transformation of <i>E.coli</i></a></h2> |
| - | <h2>The transformation of Bacillus subtilis</h2> | + | <h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/The transformation of Bacillus subtilis">The transformation of <i>Bacillus subtilis</i></a></h2> |
| - | <h1>Overlap PCR</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Overlap PCR">Overlap PCR</a></h1> |
<h1>Extracting plasmids</h1> | <h1>Extracting plasmids</h1> | ||
| - | <h2>Extracting plasmids from gram-positive bacterium</h2> | + | <h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Extracting plasmids from gram-positive bacterium">Extracting plasmids from gram-positive bacterium</a></h2> |
| - | <h2>Extracting plasmids from gram-negative bacterium<h2></div> | + | <h2><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Extracting plasmids from gram-negative bacterium">Extracting plasmids from gram-negative bacterium</a><h2></div> |
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
<div class="protocol-col-2"> | <div class="protocol-col-2"> | ||
<h>Expression of proteins</h> | <h>Expression of proteins</h> | ||
| - | <h1>Expression of proteins in Bacillus subtilis</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Expression of proteins in Bacillus subtilis">Expression of proteins in <i>Bacillus subtilis</i></a></h1> |
| - | <h1>Expression of proteins in Escherichia coli</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Expression of proteins in Escherichia coli">Expression of proteins in <i>Escherichia coli</i></a></h1> |
| - | <h1>Concentrating proteins</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Concentrating proteins">Concentrating proteins</a></h1> |
</div> | </div> | ||
<div class="protocol-col-2"> | <div class="protocol-col-2"> | ||
<h>Transdermal experiments</h> | <h>Transdermal experiments</h> | ||
<h>Immunity&Tests</h> | <h>Immunity&Tests</h> | ||
| - | <h1>ELISA</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/ELISA">ELISA</a></h1> |
| - | <h1>Sample analysis</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Sample analysis">Sample analysis</a></h1> |
</div> | </div> | ||
<div class="clear"></div> | <div class="clear"></div> | ||
<div class="protocol-col-2"> | <div class="protocol-col-2"> | ||
<h>Medium</h> | <h>Medium</h> | ||
| - | <h1>LB media</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/LB media">LB media</a></h1> |
| - | <h1>10*S base</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/10*S base">10*S base</a></h1> |
| - | <h1>HS media</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/HS media">HS media</a></h1> |
| - | <h1>LS media</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/LS media">LS media</a></h1> |
| - | <h1>2*YT media</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/2*YT media">2*YT media</a></h1> |
</div> | </div> | ||
<div class="protocol-col-2"> | <div class="protocol-col-2"> | ||
| - | <h> | + | <h>Measurement</h> |
| - | <h1>Constitutive promoter measurements</h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Constitutive promoter measurements">Constitutive promoter measurements</a></h1> |
| - | <h1> | + | <h1><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/P-lac promoter response to IPTG or Glucose">Plac promoter response to IPTG or Glucose</a></h1> |
</div> | </div> | ||
</div> | </div> | ||
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<div class="rightbar"> | <div class="rightbar"> | ||
| - | <div class="port-sidebar-border"><h> | + | <div class="port-sidebar-border"><h>Notebook</h></div> |
<div class="clear"></div> | <div class="clear"></div> | ||
| - | <div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline"> | + | <div id="t1"><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></div> |
| - | <div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols"> | + | <div id="t1"><a class="active" href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></div></div> |
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Latest revision as of 12:43, 28 October 2013
