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Team:ZJU-China/Project/GhostSensor/Perspectives
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Revision as of 14:04, 28 October 2013
Bacterial Ghost: Perspectives
Our ghost sensor platform can not only be used to detect proteins, it can also be used to specifically clear proteins in solution and achieve long-term storage of proteins.
Magic strip
For the second set of inner-membrane scaffolds, two parts of β-lactamase were fused to the periplasmic ends. When 19-mer and 25-mer biotin-modified aptamers are added to the solution and thrombin is present, aptamers act like a bridge linking streptavidin-fused membrane scaffolds and thrombin. In this way, two molecules of membrane scaffolds are pulled together and the two residuals of β-lactamase will meet each other again to become an integrity part with enzymatic activity. This activity can be detected by commercial β-lactamase testing strips, with a total preparation time of about 10 min.
β-lactamase testing strip. Left shows β-lactamase positive, right shows negative.
