Team:NYMU-Taipei/Project/Inhibition/Inhibition circuit

From 2013.igem.org

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===Circuit Regulation Testing===
===Circuit Regulation Testing===
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1. For pTet (R0040)：
+
<br>1.For pTet (R0040)：</br>
 +
  <p> pTet(R0040)+ E0840....(controll) </p>
 +
  <p> J23102+ RBS+ tetR+ Term+ pTet+ E0840 </p>
 +
  <p> J23102+ RBS+ lacI+ Term+ pTet+ E0840 </p>
 +
<br>2. For pLux/cI hybrid (R0065)：</br>
 +
  <p> pLux/cI(R0065)+ E0840....(controll) </p>
 +
  <p> J23102+ RBS+ cI+ Term+ R0065+ E0840 </p>
 +
  <p> J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840 </p>
 +
  <p> J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840</p>
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 +
===Our expectation===
 +
<br>1.</br>
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<p>(1)can see GFP under fluroscence </p>
 +
<p>(2)can not see GFP under fluroscence </p>
 +
<p>(3)can see GFP under fluroscence again</p>
 +
<br> Conclusion： tetR coding sequence has the ability to inhibit pTet.</br>
 +
 +
<br>2.</br>
 +
<p>(1)can not see GFP under fluroscence </p>
 +
<p>(2)can not see GFP under fluroscence </p>
 +
<p>(3)can see GFP under fluroscence </p>
 +
<p>(4)can not see GFP under fluroscence </p>
 +
<br> Conclusion： cI coding sequence is more dominant than LuxR/AHL, and cI coding sequence can well inhibit pLux/cI as well as LuxR& LuxI hibrid can initiate pLux/cI only when cI is absent.</br>
{{:Team:NYMU-Taipei/Footer}}
{{:Team:NYMU-Taipei/Footer}}

Revision as of 18:42, 28 October 2013

National Yang Ming University



Circuit Regulation Vedio

Circuit Regulation Testing


1.For pTet (R0040)：</br>

pTet(R0040)+ E0840....(controll)

J23102+ RBS+ tetR+ Term+ pTet+ E0840

J23102+ RBS+ lacI+ Term+ pTet+ E0840


2. For pLux/cI hybrid (R0065)：</br>

pLux/cI(R0065)+ E0840....(controll)

J23102+ RBS+ cI+ Term+ R0065+ E0840

J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840

J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840

Our expectation


1.</br>

(1)can see GFP under fluroscence

(2)can not see GFP under fluroscence

(3)can see GFP under fluroscence again


Conclusion： tetR coding sequence has the ability to inhibit pTet.</br>


2.</br>

(1)can not see GFP under fluroscence

(2)can not see GFP under fluroscence

(3)can see GFP under fluroscence

(4)can not see GFP under fluroscence


Conclusion： cI coding sequence is more dominant than LuxR/AHL, and cI coding sequence can well inhibit pLux/cI as well as LuxR& LuxI hibrid can initiate pLux/cI only when cI is absent.</br>

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