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Team:NYMU-Taipei/Project/Inhibition/Inhibition circuit
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===Circuit Regulation Testing=== | ===Circuit Regulation Testing=== | ||
| - | <br>1.For pTet (R0040)： | + | <br>1.For pTet (R0040)： |
<p> pTet(R0040)+ E0840....(controll) </p> | <p> pTet(R0040)+ E0840....(controll) </p> | ||
<p> J23102+ RBS+ tetR+ Term+ pTet+ E0840 </p> | <p> J23102+ RBS+ tetR+ Term+ pTet+ E0840 </p> | ||
<p> J23102+ RBS+ lacI+ Term+ pTet+ E0840 </p> | <p> J23102+ RBS+ lacI+ Term+ pTet+ E0840 </p> | ||
| - | <br>2. For pLux/cI hybrid (R0065)： | + | <br>2. For pLux/cI hybrid (R0065)： |
<p> pLux/cI(R0065)+ E0840....(controll) </p> | <p> pLux/cI(R0065)+ E0840....(controll) </p> | ||
<p> J23102+ RBS+ cI+ Term+ R0065+ E0840 </p> | <p> J23102+ RBS+ cI+ Term+ R0065+ E0840 </p> | ||
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===Our expectation=== | ===Our expectation=== | ||
| - | <br>1. | + | <br>1. |
<p>(1)can see GFP under fluroscence </p> | <p>(1)can see GFP under fluroscence </p> | ||
<p>(2)can not see GFP under fluroscence </p> | <p>(2)can not see GFP under fluroscence </p> | ||
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<br> Conclusion： tetR coding sequence has the ability to inhibit pTet.</br> | <br> Conclusion： tetR coding sequence has the ability to inhibit pTet.</br> | ||
| - | <br>2. | + | <br>2. |
<p>(1)can not see GFP under fluroscence </p> | <p>(1)can not see GFP under fluroscence </p> | ||
<p>(2)can not see GFP under fluroscence </p> | <p>(2)can not see GFP under fluroscence </p> | ||
Revision as of 18:43, 28 October 2013
Circuit Regulation Vedio
Circuit Regulation Testing
1.For pTet (R0040)：
pTet(R0040)+ E0840....(controll)
J23102+ RBS+ tetR+ Term+ pTet+ E0840
J23102+ RBS+ lacI+ Term+ pTet+ E0840
2. For pLux/cI hybrid (R0065)：
pLux/cI(R0065)+ E0840....(controll)
J23102+ RBS+ cI+ Term+ R0065+ E0840
J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840
J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840
Our expectation
1.
(1)can see GFP under fluroscence
(2)can not see GFP under fluroscence
(3)can see GFP under fluroscence again
Conclusion： tetR coding sequence has the ability to inhibit pTet.</br>
2.
(1)can not see GFP under fluroscence
(2)can not see GFP under fluroscence
(3)can see GFP under fluroscence
(4)can not see GFP under fluroscence
Conclusion： cI coding sequence is more dominant than LuxR/AHL, and cI coding sequence can well inhibit pLux/cI as well as LuxR& LuxI hibrid can initiate pLux/cI only when cI is absent.</br>
