Team:TU-Delft

From 2013.igem.org

(Difference between revisions)
 
(86 intermediate revisions not shown)
Line 3: Line 3:
{{:Team:TU-Delft/Templates/Frog}}
{{:Team:TU-Delft/Templates/Frog}}
{{:Team:TU-Delft/Templates/Logo}}
{{:Team:TU-Delft/Templates/Logo}}
-
 
-
 
-
 
-
<div style="margin-left:30px;margin-right:30px; width:900px;float:left;"> 
 
-
<h2 align="center">Peptidor</h2>
 
<html>
<html>
 +
 +
<div style="margin-top:-20px;margin-bottom:5px;">
 +
<center>
 +
<a href="https://static.igem.org/mediawiki/2013/8/82/Pep9.jpg">
 +
<img src="https://static.igem.org/mediawiki/2013/8/82/Pep9.jpg" alt=""  HEIGHT=90" WIDTH="700" />
 +
</a>
 +
</center>
 +
</div>
<!--copyright METU TEAM iGem 2012-->
<!--copyright METU TEAM iGem 2012-->
Line 66: Line 69:
<ul>
<ul>
    <li>
    <li>
-
    <h2>Who are we?</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">We are the iGEM 2013 team of TU Delft, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant <i>Staphylococcus aureus, MRSA</i>.</p>
+
    <h2>Who are we?</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant <i>Staphylococcus aureus, MRSA</i>.</p>
    </li>
    </li>
 +
 +
<li> <h2>Aim</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> To engineer <i>Escherichia coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.
 +
</p>
 +
 +
                                     
 +
    </li>
 +
    <li>
    <li>
    <h2>Sensing autoinducing peptides</h2>   
    <h2>Sensing autoinducing peptides</h2>   
Line 77: Line 87:
    <li>
    <li>
    <h2>Peptide Production</h2>
    <h2>Peptide Production</h2>
-
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> <i>E.coli</i> detects <i>Staphylococcus aureus</i> by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are  being produced </p>
+
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> <i>E. coli</i> detects <i>Staphylococcus aureus</i> by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are  being produced </p>
    </li>
    </li>
      <li>
      <li>
Line 90: Line 100:
In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.</p>  
In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.</p>  
    </li>
    </li>
-
       
+
   
 +
<li>
 +
    <h2>Zephyr</h2>
 +
<div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify">
 +
Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.
 +
</p>
 +
    </li>
 +
   
 +
 
 +
   
</ul>
</ul>
</div>
</div>
Line 100: Line 119:
</a>
</a>
                                     </li>
                                     </li>
 +
 +
<li>
 +
<a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview">
 +
<img src="https://static.igem.org/mediawiki/2013/5/51/Schem-new.jpg" alt="" HEIGHT="348" WIDTH="600"/>
 +
</a>
 +
 +
</li>
 +
 +
    <li>
    <li>
<a href="https://2013.igem.org/Team:TU-Delft/Sensing">
<a href="https://2013.igem.org/Team:TU-Delft/Sensing">
Line 119: Line 147:
<a href="https://2013.igem.org/Team:TU-Delft/Killswitch">
<a href="https://2013.igem.org/Team:TU-Delft/Killswitch">
<img src="https://static.igem.org/mediawiki/2013/f/f8/Kill2.jpg" alt="" HEIGHT="348" WIDTH="600"/>
<img src="https://static.igem.org/mediawiki/2013/f/f8/Kill2.jpg" alt="" HEIGHT="348" WIDTH="600"/>
 +
</a></li>
 +
 +
<li>
 +
<a href="https://2013.igem.org/Team:TU-Delft/Zephyr">
 +
<img src="https://static.igem.org/mediawiki/2013/4/4c/2D_table_ZephyrHome.jpg" alt="" WIDTH="600"/>
</a>
</a>
 +
</li>
</li>
Line 127: Line 161:
<div class="slider-nav">
<div class="slider-nav">
<a href="https://2013.igem.org/Team:TU-Delft/Team">1</a>
<a href="https://2013.igem.org/Team:TU-Delft/Team">1</a>
-
<a href="https://2013.igem.org/Team:TU-Delft/Sensing">2</a>
+
                              <a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview">2</a>
-
<a href="https://2013.igem.org/Team:TU-Delft/Peptides">3</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Sensing">3</a>
-
<a href="https://2013.igem.org/Team:TU-Delft/Timer">4</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Peptides">4</a>
-
                                 <a href="https://2013.igem.org/Team:TU-Delft/Killswitch">5</a>
+
<a href="https://2013.igem.org/Team:TU-Delft/Timer">5</a>
 +
                                 <a href="https://2013.igem.org/Team:TU-Delft/Killswitch">6</a>
 +
<a href="https://2013.igem.org/Team:TU-Delft/Zephyr">7</a>
<div class="cl">&nbsp;</div>
<div class="cl">&nbsp;</div>
Line 140: Line 176:
<!-- Main -->
<!-- Main -->
<br>
<br>
 +
<div style="margin-left:40px;width:420px;float:left;display:inline-block;">   
<div style="margin-left:40px;width:420px;float:left;display:inline-block;">   
 +
<br>
 +
<script>(function(d, s, id) {
<script>(function(d, s, id) {
Line 165: Line 204:
<br>
<br>
<br>
<br>
-
<br>
+
 
-
<br>
+
<center>
-
<br>
+
<p> <h3> Check out what news in Netherlands has to say about the achievements of our Team </p><a href="https://2013.igem.org/Team:TU-Delft/PR" target="blank"> In News. </a> </h3> <br> </center>
-
<br>
+
<div style="margin-top:10px;margin-left:400px;float:left;display:inline-block;">   
<div style="margin-top:10px;margin-left:400px;float:left;display:inline-block;">   
Line 218: Line 256:
<td style="text-align: center;">
<td style="text-align: center;">
</td>
</td>
 +
</tr>
 +
 +
<tr>
 +
<td style="text-align: center;" width="250px">
 +
<a href="https://static.igem.org/mediawiki/2013/archive/5/58/20131028223200!Logo_ERASynBio.png"target="_blank"><img src=" https://static.igem.org/mediawiki/2013/archive/5/58/20131028223200!Logo_ERASynBio.png"width=120 height=40> </a>
 +
</td>
</tr>
</tr>
</table>
</table>

Latest revision as of 22:37, 28 October 2013


  • Who are we?

    We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant Staphylococcus aureus, MRSA.

  • Aim

    To engineer Escherichia coli that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.

  • Sensing autoinducing peptides

    The sensing part of the of Staphylococcus aureus Agr system is engineered in E. coli. In that way E. coli detects the AIPs that are produced by Staphylococcus aureus which induces the peptide production.

  • Peptide Production

    E. coli detects Staphylococcus aureus by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced

  • Timer

    With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.

  • Kill Switch

    In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.

  • Zephyr

    Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.

 
1 2 3 4 5 6 7
 



Locations of visitors to this page Free Web Counters


Check out what news in Netherlands has to say about the achievements of our Team

In News.


Our sponsors

Retrieved from "http://2013.igem.org/Team:TU-Delft"