"
Team:TU-Delft
From 2013.igem.org
| (37 intermediate revisions not shown) | |||
| Line 5: | Line 5: | ||
| - | <html> | + | <html> |
| - | <div style="margin-top:- | + | <div style="margin-top:-20px;margin-bottom:5px;"> |
<center> | <center> | ||
| - | <a href="https://static.igem.org/mediawiki/2013/ | + | <a href="https://static.igem.org/mediawiki/2013/8/82/Pep9.jpg"> |
| - | <img src="https://static.igem.org/mediawiki/2013/ | + | <img src="https://static.igem.org/mediawiki/2013/8/82/Pep9.jpg" alt="" HEIGHT=90" WIDTH="700" /> |
| - | </a | + | </a> |
</center> | </center> | ||
</div> | </div> | ||
| - | |||
<!--copyright METU TEAM iGem 2012--> | <!--copyright METU TEAM iGem 2012--> | ||
| Line 53: | Line 52: | ||
margin-left:10px; | margin-left:10px; | ||
} | } | ||
| + | |||
| + | |||
| Line 61: | Line 62: | ||
</head> | </head> | ||
| - | |||
| - | |||
<!-- Slider --> | <!-- Slider --> | ||
<div id="slider"> | <div id="slider"> | ||
| Line 73: | Line 72: | ||
</li> | </li> | ||
| + | |||
| + | <li> <h2>Aim</h2><div style="margin-left:10px;margin-right:10px;float:left;display:inline-block;"><p align="justify"> To engineer <i>Escherichia coli</i> that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules. | ||
| + | </p> | ||
| + | |||
| + | |||
| + | </li> | ||
| + | |||
<li> | <li> | ||
<h2>Sensing autoinducing peptides</h2> | <h2>Sensing autoinducing peptides</h2> | ||
| Line 113: | Line 119: | ||
</a> | </a> | ||
</li> | </li> | ||
| + | |||
| + | <li> | ||
| + | <a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview"> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/5/51/Schem-new.jpg" alt="" HEIGHT="348" WIDTH="600"/> | ||
| + | </a> | ||
| + | |||
| + | </li> | ||
| + | |||
| + | |||
<li> | <li> | ||
<a href="https://2013.igem.org/Team:TU-Delft/Sensing"> | <a href="https://2013.igem.org/Team:TU-Delft/Sensing"> | ||
| Line 146: | Line 161: | ||
<div class="slider-nav"> | <div class="slider-nav"> | ||
<a href="https://2013.igem.org/Team:TU-Delft/Team">1</a> | <a href="https://2013.igem.org/Team:TU-Delft/Team">1</a> | ||
| - | <a href="https://2013.igem.org/Team:TU-Delft/Sensing"> | + | <a href="https://2013.igem.org/Team:TU-Delft/ProjectOverview">2</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Peptides"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Sensing">3</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Timer"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Peptides">4</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Killswitch"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Timer">5</a> |
| - | <a href="https://2013.igem.org/Team:TU-Delft/Zephyr"> | + | <a href="https://2013.igem.org/Team:TU-Delft/Killswitch">6</a> |
| + | <a href="https://2013.igem.org/Team:TU-Delft/Zephyr">7</a> | ||
<div class="cl"> </div> | <div class="cl"> </div> | ||
| Line 159: | Line 175: | ||
<!-- End Slider --> | <!-- End Slider --> | ||
<!-- Main --> | <!-- Main --> | ||
| + | <br> | ||
| + | |||
| + | <div style="margin-left:40px;width:420px;float:left;display:inline-block;"> | ||
| + | <br> | ||
| Line 184: | Line 204: | ||
<br> | <br> | ||
<br> | <br> | ||
| - | < | + | |
| - | < | + | <center> |
| - | < | + | <p> <h3> Check out what news in Netherlands has to say about the achievements of our Team </p><a href="https://2013.igem.org/Team:TU-Delft/PR" target="blank"> In News. </a> </h3> <br> </center> |
| - | <br> | + | |
<div style="margin-top:10px;margin-left:400px;float:left;display:inline-block;"> | <div style="margin-top:10px;margin-left:400px;float:left;display:inline-block;"> | ||
| Line 237: | Line 256: | ||
<td style="text-align: center;"> | <td style="text-align: center;"> | ||
</td> | </td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td style="text-align: center;" width="250px"> | ||
| + | <a href="https://static.igem.org/mediawiki/2013/archive/5/58/20131028223200!Logo_ERASynBio.png"target="_blank"><img src=" https://static.igem.org/mediawiki/2013/archive/5/58/20131028223200!Logo_ERASynBio.png"width=120 height=40> </a> | ||
| + | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Latest revision as of 22:37, 28 October 2013
-
Who are we?
We are the iGEM 2013 team of Delft University of Technology, Netherlands. We are working on the production of antimicrobial peptides in order to kill methicillin resistant Staphylococcus aureus, MRSA.
Aim
To engineer Escherichia coli that can detect MRSA in order to locally produce and deliver antimicrobial peptides. The project is divided into several essential modules.
Sensing autoinducing peptides
The sensing part of the of Staphylococcus aureus Agr system is engineered in E. coli. In that way E. coli detects the AIPs that are produced by Staphylococcus aureus which induces the peptide production.
Peptide Production
E. coli detects Staphylococcus aureus by its own quorum sensing system, and at that moment inactivated antimicrobial peptides are being produced
Timer
With the use of a timer the peptide will be activated by cleavage of an inactivating tag. With the use of a timer, high concentrations of peptide can be delivered very locally in order to efficiently kill MRSA.
Kill Switch
In order to ensure the safety of the system, the peptide activating protease is combined with a kill switch that will kill our E. coli.
Zephyr
Zephyr is a low-cost Do It Yourself (DIY) machine which can scan petridishes and 96 well plates for expression of fluorescence at micrometer scale.
Check out what news in Netherlands has to say about the achievements of our Team In News.
Our sponsors
