Latest revision as of 03:28, 29 October 2013

USTC_China

In Situ Transdermal Vaccine

2013, We Redefined Vaccine
A Revolutionary Transdermal Vaccine
Safe, Reliable, Needle-free and Available for Everyone

Motivation

We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
more

Design of Project

With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered Bacillus subtilis; besides, we designed a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.

ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will make up great percentage of
in the total bacteria in our "band-aid".
more

LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
more

TNFα
TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
more

REPORTER
Reporter notifies users the status of vaccine patch and when to stick. Furthermore, it contains killing switch as a biosafety guard.
more

Experimental Measurement

Construction of Vectors
We took E.coli and the pET vector as the positive control and realized our ideas in the

B.subtilis

expression system based on pHT43 shuttle vector.
more

Tests on fusion protein
To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.
more

Immunogenicity experiments
The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
more

Team:USTC CHINA

From 2013.igem.org

Latest revision as of 03:28, 29 October 2013

In Situ Transdermal Vaccine

Motivation

Design of Project

Experimental Measurement

Modeling

Suicide mechanism of the reporter system

The optimal culture conditions of Bacillus subtilis

Designs of Immune Experiments

Human Practice

Communication

Activity

Thanks to the sponsors

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+    <ul id="nav">
+        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
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+        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
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+            <ul class="subs">
-                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project" target="_blank">Project</a>
+                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
-                    <ul class="subs">
+                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview" target="_blank">Overview</a></li>
+                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails" target="_blank">Project Details</a></li>
+                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results" target="_blank">Results</a></li>
+                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
-                    </ul>
+             </ul>
-                </li>
+        </li>
-                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook" target="_blank">Notebook</a>
+        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
-                    <ul class="subs">
+              <ul class="subs">
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline" target="_blank">Timeline</a></li>
+                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols" target="_blank">Protocols</a></li>
+                 <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
-                    </ul>
+              </ul>
-                </li>
+        </li>
-                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/" target="_blank">Modeling</a>
+        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
-                    <ul class="subs">
+              <ul class="subs">
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem" target="_blank">Reporter System</a></li>
+                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture" target="_blank">B.Subtilis Culture</a></li>
+                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling" target="_blank">Mice Modeling</a></li>
+                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
-                    </ul>
+              </ul>
-              </li>
+        </li>
-               <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice" target="_blank">Human Practice</a>
+        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
-                    <ul class="subs">
+              <ul class="subs">
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">Communication</a></li>
+                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity" target="_blank">Activity</a></li>
+                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
-                    </ul>
+              </ul>
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+        </li>
-                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team" target="_blank">Team</a>
+        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
-                <ul class="subs">
+              <ul class="subs">
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team/Members" target="_blank">Members</a></li>
+                   <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
-                        <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA" target="_blank">Profile</a></li>
+                   <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
-                        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team/Attribute" target="_blank">Attribute</a></li>
+                   <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
-                  </ul>
+                   <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
-              </li>
+              </ul>
-                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
+         </li>
-            </ul>
+         <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
-  </div></div>
+     </ul>
+     <div id="igemlogo" style="float:right;margin: -48px 20px 0px 0px;">
+          <a href="https://2013.igem.org/Main_Page" target="_blank"><img src="https://static.igem.org/mediawiki/2013/2/26/2013ustcigem_IGEM_basic_Logo.png" alt="igem home page" width="50" height="40" /></a>
+      </div>
+   </div>
+</div>
 <div class="content" align="center">
-<div class="pic-bar">
+  <div class="pic-bar">
-<div class="home-pic">
+    <div class="home-pic">
-<div class="conbar1">
+      <div class="conbar1">
-<div id="breadcrumb"></div></div>
+        <div id="breadcrumb"></div>
-<div class="page-tittle" align="right">
+      </div>
-<h1>In Situ Transdermal Vaccine</h1></div>
+      <div class="page-tittle" align="right">
-<div class="page-para" align="right">
+        <h1>In Situ <span>T</span>ransdermal <span>Vaccine</span></h1>
-<p>Fresh Expression, No Purification<br />Standardization, Block-based design<br />Easy-transportation, Low Storage External Costs<br />
+      </div>
-Almost No Demand For Medical Conditions And Proferssionals<br /></p>
+      <div class="page-para" align="right">
-<p></p><p><a class="button-home" href="https://2013.igem.org/Team:USTC_CHINA/Project"><span>Project Details</span></a></p></div>
+        <p>2013, We Redefined Vaccine<br />A Revolutionary Transdermal Vaccine<br />Safe, Reliable, Needle-free and Available for Everyone<br />
-</div></div></div>
+        </p><p></p>
+        <p>
+            <a class="button-home" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview"><span>Project </span></a>
+        </p>
+      </div>
+    </div>
+  </div>
+</div>
 <div class="content" id="homecol" align="center">
@@ Line 74: / Line 87: @@
 <div id="home" class="leftbar" align="left">
 <div id="abstract-bar">
-<h1>Why We Begin</h1>
+<h1>Motivation</h1>
 <div id="abs-1">
    <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
    <div id="abs-text">
-   we hope to provide a needle-free and non-skin-damage vaccination,
+   We hope to develop a fresh-expression transdermal vaccination.
-   <br />we hope everyone, even in the least developed area, could enjoy the benefit of medical progress.<br />
+   <br />We hope everyone in this world could equally enjoy the benefit of medical progress.<br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">more about project background</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" /></div>
 </div>
 <div id="pro-bar">
-<h1>How We Design</h1>
+<h1>Design of Project</h1>
    <div id="pro-text">
-   With the support of <span>TD-1</span>, a special polypeptide, which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a kill switch in the fouth type B.subtilis to ensure biosafety.</div>
+   With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered <i>Bacillus subtilis</i>; besides, we designed a reporter system, including a kill switch, in the fouth type <i>B.subtilis</i> to improve usability and ensure biosafety.</div>
 <div id="ls" class="pro-col">
    <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
    <div id="pro-de-text">
    <span>ANTIGEN</span><br />
-As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will share a great percentage of<br /> the total bacteria in our "band-aid".
+As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will make up great percentage of<br />in the total bacteria in our "band-aid".
 <br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 103: / Line 117: @@
    <div id="pro-de-text">
    <span>LTB</span><br />
-As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.
+As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
 <br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 112: / Line 126: @@
    <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
    <div id="pro-de-text">
-   <span>KNFα</span><br />
+   <span>TNFα</span><br />
-It's worth mentioning that to improve the effects of <br />traditional vaccine, as another kind of our TD1-adjuvant expresser, KNFα bear the weight of our hope to increase the sensitivity of our immune system.
+TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
 <br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 123: / Line 137: @@
    <div id="pro-de-text">
    <span>REPORTER</span><br />
-Reporter, which notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms .  Further more killing switch is existed in them as a biosafety guard.
+Reporter notifies users the status of vaccine patch and when to stick.  Furthermore, it contains killing switch as a biosafety guard.
 <br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 132: / Line 146: @@
 <div id="exp-bar">
-<h1>What We've Achived</h1>
+<h1>Experimental Measurement</h1>
 <div class="exp-col">
    <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
    <div id="exp-text">
-  <span>Molecules part</span><br />
+  <span>Construction of Vectors</span><br />
-We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.<br />
+We took <i>E.coli</i> and the pET vector as the positive control and realized our ideas in the <div class="oblique">B.subtilis</div> expression system based on pHT43 shuttle vector.<br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 145: / Line 159: @@
    <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
    <div id="exp-text">
-   <span>Protein part</span><br />
+   <span>Tests on fusion protein</span><br />
-   Before we achieve fresh expression, we need first use purified protein to obtain exact data to verify our assumption.<br />
+   To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.<br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Results">more about project results</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 154: / Line 168: @@
    <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" />
    <div id="exp-text">
-   <span>Mice experiments</span><br />
+   <span>Immunogenicity experiments</span><br />
-   The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments<br />
+   The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments<br />
-   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>
+   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">more</a>
    </div>
    <img class="img-right" src="https://static.igem.org/mediawiki/2013/8/8e/2013ustc-china_99.png" width="16" height="13" />
@@ Line 165: / Line 179: @@
 </div>
-<div id="home" class="rightbar" align="left">
+<div id="home" class="rightbar" style="position:static;" align="left">
 <div id="modle-bar">
-<h1>Modleing</h1>
+<h1>Modeling</h1>
 <div id="modle-col">
-<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">Suicide mechanism of the reporter system</a></h2>
+<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Suicide mechanism of the reporter system</a></h2>
-<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture"><img src="images/model1.png" width="310" height="180" /></a>
+<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch"><img src="https://static.igem.org/mediawiki/2013/4/48/2013ustc-china_Model1.png" width="310" height="160" /></a>
 <br />
-<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem">The optimal culture conditions of Bacillus subtilis</a></h2>
+<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">The optimal culture conditions of Bacillus subtilis</a></h2>
-<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/ReporterSystem"><img src="images/model2.png" width="310" height="264" /></a>
+<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture"><img src="https://static.igem.org/mediawiki/2013/1/10/2013ustc-china_Model2.png" width="310" height="234" /></a>
-<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/MiceModeling">
+<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">
-Mice Modeling</a></h2>
+Designs of Immune Experiments</a></h2>
 </div>
 </div>
@@ Line 183: / Line 197: @@
 <h1>Human Practice</h1>
 <div id="hum-col">
-<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">
+<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
 Communication</a></h2>
-<a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank"><img src="images/china-map.png" width="310" height="259" /></a>
+<a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication"><img src="https://static.igem.org/mediawiki/2013/5/5e/2013ustc-china_China-map.png" width="310" height="259" /></a>
-<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" target="_blank">
+<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
 Activity</a></h2>
 </a>
@@ Line 202: / Line 216: @@
      <div class="sponsor" id="db">
        <p align="center">Deutsche Bank, China</p>
-         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="images/USTC-Software-images-db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
+         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="https://static.igem.org/mediawiki/2013/a/a7/2013ustc-china_Db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
          </div>
      </div>
@@ Line 208: / Line 222: @@
       <div class="sponsor">
          <p align="center">School of Life Sciences, USTC</p>
-         <div align="center"><a href="http://en.biox.ustc.edu.cn/" target="_blank"><img src="images/USTC-Software-images-life-logo.png" alt="School of Life Sciences, USTC" height="65px" align="absmiddle" width="280px"></a>
+         <div align="center"><a href="http://en.biox.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/98/2013ustc-china_Life-logo.png" alt="School of Life Sciences, USTC" height="65px" align="absmiddle" width="280px"></a>
          </div>
      </div>
@@ Line 214: / Line 228: @@
      <div class="sponsor">
        <p align="center">USTC<span style="color:#fcc644">IF</span></p>
-         <div align="center"><a href="http://www.ustcif.org/" target="_blank"><img src="images/USTC-Software-images-ustcif-logo.png" height="65px" align="absmiddle" width="280px"></a>
+         <div align="center"><a href="http://www.ustcif.org/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/3c/2013ustc-china_Ustcif-logo.png" height="65px" align="absmiddle" width="280px"></a>
          </div>
      </div>
@@ Line 220: / Line 234: @@
      <div class="sponsor">
          <p align="center">School of the Gifted Young, USTC</p>
-         <div align="center"><a href="http://en.scgy.ustc.edu.cn/" target="_blank"><img src="images/Gifted_young.jpg" alt="School of the Gifted Young, USTC" height="65px" align="absmiddle" width="280px"></a>
+         <div align="center"><a href="http://en.scgy.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/c/c1/2013ustc-china_Gifted_young.jpg" height="65px" align="absmiddle" width="280px"></a>
          </div>
      </div>
@@ Line 226: / Line 240: @@
      <div class="sponsor">
          <p align="center">School of physics, USTC</p>
-         <div align="center"><a href="http://en.physics.ustc.edu.cn/" target="_blank"><img src="images/USTC-Software-images-physics-logo.png" height="65px" align="absmiddle" width="280px"></a>
+         <div align="center"><a href="http://en.physics.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/6/68/2013ustc-china_Physics-logo.png" height="65px" align="absmiddle" width="280px"></a>
          </div>
      </div>
@@ Line 232: / Line 246: @@
      <div class="sponsor">
          <p align="center">Teaching Affairs Office of USTC</p>
-         <div align="center"><a href="http://www.teach.ustc.edu.cn/" target="_blank"><img src="images/USTC-Software-images-teaching-logo.png" alt="Teaching Affairs Office Of USTC" style="background-color:white" height="65px" align="absmiddle" width="280px"></a>
+         <div align="center"><a href="http://www.teach.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/f/f9/2013ustc-china_Teaching-logo.png" alt="Teaching Affairs Office Of USTC" style="background-color:white" height="65px" align="absmiddle" width="280px"></a>
          </div>
      </div>