<li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails">Project Details</a></li>

         <h1>In Situ Transdermal Vaccine</h1>

         <p>Fresh Expression, No Purification<br />Standardization, Block-based design<br />Easy-transportation, Low Storage External Costs<br />Almost No Demand For Medical Conditions And Proferssionals<br />

<h1>Why We Begin</h1>

   We hope to provide a needle-free and non-skin-damage vaccination,

   <br />we hope everyone, even in the least developed area, could enjoy the benefit of medical progress.<br />

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background">more about project background</a>

<h1>How We Design</h1>

   With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Background#" target="_blank">TD-1</a></span>, a special polypeptide, which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.</div>

As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will share a great percentage of<br /> the total bacteria in our "band-aid".

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>

As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>

It's worth mentioning that to improve the effects of <br />traditional vaccine, as another kind of our TD1-adjuvant expresser, KNFα bear the weight of our hope to increase the sensitivity of our immune system.

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>

Reporter, which notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms .  Further more killing switch is existed in them as a biosafety guard.

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Design">more about project design</a>

<h1>What We've Achieved</h1>

  <span>Molecules part</span><br />

We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.<br />

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more about project results</a>

   <span>Protein part</span><br />

   Before we achieve fresh expression, we need first use purified protein to obtain exact data to verify our assumption.<br />

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/ProjectDetails/Results">more about project results</a>

   <span>Mice experiments</span><br />

   The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments<br />

   <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">more about project results</a>

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