Team:USTC CHINA
From 2013.igem.org
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<div class="page-tittle" align="right"> | <div class="page-tittle" align="right"> | ||
- | <h1>In Situ | + | <h1>In Situ <span>T</span>ransdermal <span>Vaccine</span></h1> |
</div> | </div> | ||
<div class="page-para" align="right"> | <div class="page-para" align="right"> | ||
- | <p> | + | <p>2013, We Redefined Vaccine<br />A Revolutionary Transdermal Vaccine<br />Safe, Reliable, Needle-free and Available for Everyone<br /> |
</p><p></p> | </p><p></p> | ||
<p> | <p> | ||
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<h1>Design of Project</h1> | <h1>Design of Project</h1> | ||
<div id="pro-text"> | <div id="pro-text"> | ||
- | With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery | + | With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered <i>Bacillus subtilis</i>; besides, we designed a reporter system, including a kill switch, in the fouth type <i>B.subtilis</i> to improve usability and ensure biosafety.</div> |
<div id="ls" class="pro-col"> | <div id="ls" class="pro-col"> | ||
<img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | ||
<div id="pro-de-text"> | <div id="pro-de-text"> | ||
<span>ANTIGEN</span><br /> | <span>ANTIGEN</span><br /> | ||
- | As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will | + | As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will make up great percentage of<br />in the total bacteria in our "band-aid". |
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<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | ||
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<div id="pro-de-text"> | <div id="pro-de-text"> | ||
<span>LTB</span><br /> | <span>LTB</span><br /> | ||
- | As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a | + | As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine. |
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<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | ||
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<div id="pro-de-text"> | <div id="pro-de-text"> | ||
<span>TNFα</span><br /> | <span>TNFα</span><br /> | ||
- | + | TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses. | |
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<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | ||
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<div id="pro-de-text"> | <div id="pro-de-text"> | ||
<span>REPORTER</span><br /> | <span>REPORTER</span><br /> | ||
- | Reporter notifies users | + | Reporter notifies users the status of vaccine patch and when to stick. Furthermore, it contains killing switch as a biosafety guard. |
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<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a> | ||
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<img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | ||
<div id="exp-text"> | <div id="exp-text"> | ||
- | <span> | + | <span>Construction of Vectors</span><br /> |
- | We took E.coli and the | + | We took <i>E.coli</i> and the pET vector as the positive control and realized our ideas in the <div class="oblique">B.subtilis</div> expression system based on pHT43 shuttle vector.<br /> |
<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a> | ||
</div> | </div> | ||
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<img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | ||
<div id="exp-text"> | <div id="exp-text"> | ||
- | <span> | + | <span>Tests on fusion protein</span><br /> |
- | + | To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.<br /> | |
<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a> | ||
</div> | </div> | ||
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<img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | <img class="img-left" src="https://static.igem.org/mediawiki/2013/0/02/2013ustc-china_66.png" width="16" height="14" /> | ||
<div id="exp-text"> | <div id="exp-text"> | ||
- | <span> | + | <span>Immunogenicity experiments</span><br /> |
- | The most | + | The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments<br /> |
<a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">more</a> | <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">more</a> | ||
</div> | </div> |
Latest revision as of 03:28, 29 October 2013