<p>Fresh Expression, No Purification<br />Standardized, Block-based Design<br />Easy-transportation, Low Storage External Costs<br />Almost No Demand For Medical Conditions And Proferssionals<br />

   With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery through intact skin, we construct three different fuctional engineered bacillus subtilis; besides, we design a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.</div>

As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will share a great percentage of<br /> the total bacteria in our "band-aid".

As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a wild using adjuvant for most of exsiting injected vaccine.

TNFαis a chemotactic factor that recruits Langerhans Cells, antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for immigrating LC and triggering more intense immune responses.

Reporter notifies users whether the status of vaccine patch is all right and when they can stick the patches to arms .  Further more killing switch is conveyed in them as a biosafety guard.

  <span>Molecules part</span><br />

We took E.coli and the PET vector as the positive control and realized our ideas in the B.subtilis expression system based on PHT43 shuttle vector.<br />

   <span>Protein part</span><br />

   Before we achieve fresh expression, we use purified protein to obtain accurate data to verify our assumptions.<br />

   <span>Mice experiments</span><br />

   The most obvious character of our 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also tried mice experiments, which could be divided into transdermal experiments in vitro and animate mice experiments<br />