Team:USTC CHINA

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        <li class="active"><a href="https://2013.igem.org/Team:USTC_CHINA">Home</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Project</a>
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            <ul class="subs">
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview">Overview</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">Background</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">Design</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">Results</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Parts">Parts</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook">Notebook</a>
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              <ul class="subs">
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Timeline">Timeline</a></li>
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                <li><a href="https://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols">Protocols</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/">Modeling</a>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Kill Switch</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">B.Subtilis Culture</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">Designs of Immune Experiments</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice">Human Practice</a>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication" >Communication</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Activity">Activity</a></li>
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              </ul>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Team</a>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Team">Members</a></li>
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                  <li><a href="https://igem.org/Team.cgi?year=2013&team_name=USTC_CHINA">Profile</a></li>
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                  <li><a href="https://2013.igem.org/Team:USTC_CHINA/Attributions">Attributions</a></li>
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                  <li><a href="https://igem.org/2013_Judging_Form?id=1074#iGEM_Medals">Achievements</a></li>
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        <li><a href="https://2013.igem.org/Team:USTC_CHINA/Safety">Safety</a></li>
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        <h1>In Situ <span>T</span>ransdermal <span>Vaccine</span></h1>
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        <p>2013, We Redefined Vaccine<br />A Revolutionary Transdermal Vaccine<br />Safe, Reliable, Needle-free and Available for Everyone<br />
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        </p><p></p>
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        <p>
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            <a class="button-home" href="https://2013.igem.org/Team:USTC_CHINA/Project/Overview"><span>Project </span></a>
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<h1>Motivation</h1>
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  We hope to develop a fresh-expression transdermal vaccination.
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  <br />We hope everyone in this world could equally enjoy the benefit of medical progress.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background">more</a>
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<h1>Design of Project</h1>
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  With the support of <span><a href="https://2013.igem.org/Team:USTC_CHINA/Project/Background#" target="_blank">TD-1</a></span>, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered <i>Bacillus subtilis</i>; besides, we designed a reporter system, including a kill switch, in the fouth type <i>B.subtilis</i> to improve usability and ensure biosafety.</div>
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  <span>ANTIGEN</span><br />
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As the core of our in-situ system,<br /> TD1-antigen expresser is designed<br /> and will make up great percentage of<br />in the total bacteria in our "band-aid".
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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  <span>LTB</span><br />
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As one kind of our adjuvant expresser,<br />TD1-LTB is used to enhance the antigenicity.<br /> For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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  <span>TNFα</span><br />
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TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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  <span>REPORTER</span><br />
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Reporter notifies users the status of vaccine patch and when to stick.  Furthermore, it contains killing switch as a biosafety guard.
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Design">more</a>
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<h1>Experimental Measurement</h1>
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<span>Construction of Vectors</span><br />
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We took <i>E.coli</i> and the pET vector as the positive control and realized our ideas in the <div class="oblique">B.subtilis</div> expression system based on pHT43 shuttle vector.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
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  <span>Tests on fusion protein</span><br />
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  To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results">more</a>
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  <span>Immunogenicity experiments</span><br />
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  The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments<br />
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  <a href="https://2013.igem.org/Team:USTC_CHINA/Project/Results/FurthurWork">more</a>
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<h1>Modeling</h1>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch">Suicide mechanism of the reporter system</a></h2>
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                <span style="display: none;" class="bottom"><strong>We are USTC_CHINA.Welcome to our lab!</strong><br>
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<a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/KillSwitch"><img src="https://static.igem.org/mediawiki/2013/4/48/2013ustc-china_Model1.png" width="310" height="160" /></a>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/B.SubtilisCulture">The optimal culture conditions of Bacillus subtilis</a></h2>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/Modeling/DesignsofImmuneExperiments">
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                <span style="display: none;" class="bottom"><strong>Our pipettes</strong><br>
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Designs of Immune Experiments</a></h2>
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<div id="content1p">Project Description</div>
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<h1>Human Practice</h1>
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<div id="content2p"><p>Based on the fundamental principles and technology of synthetic biology, our team aims at designing a new immune system containing transdermal peptide that expresses genes and produces immune responses simultaneously. To reach this goal, we need to build four kinds of engineered bacteria and find out effective immune responses. Meanwhile, an appropriate reporting system guiding vaccination at different stages is also required to meet the demand of practical, safe and human-friendly production. The Bacillus subtilis we use is WB800N, which is modified into four types of engineered bacteria, combined with the gene coding LTB, HBsAg, reporter, and LC Luman-recruiting factor respectively, each of which contains the gene coding transdermal peptide. Then, the engineered bacteria will produce mixture of fusion protein in the skin and activate immune responses.</p>
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<p> The introducing of transdermal peptide, together with the simultaneous immune response with protein expression, has granted our work great advantages over other existing immune system, both in economy and operation. Therefore it is expected to become a promising new vaccine research field.</P>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
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<p> So far, we have successfully synthesize four kinds of plasmids, and the sequences of them all have been proved correct. Additionally, we have managed to transform three plasmids from E. coli to Bacillus subtilis, leaving GFP in progress. </p>
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Communication</a></h2>
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  <p> For the coming days, we plan to continue our transformation and experiment on the mice to check the effects of our vaccine, whereas other tasks, such as human practice and the standardization of our parts, are in our future schedule.</p></div>
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<a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication"><img src="https://static.igem.org/mediawiki/2013/5/5e/2013ustc-china_China-map.png" width="310" height="259" /></a>
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<h2><a href="https://2013.igem.org/Team:USTC_CHINA/HumanPractice/Communication">
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     <h3 id="sponsors-title" align="center"><em>Thanks to the sponsors</em></h3><br>
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       <p align="center">Deutsche Bank, China</p>
       <p align="center">Deutsche Bank, China</p>
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         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="https://static.igem.org/mediawiki/2012/f/fc/USTC-Software-images-db-logo.png" alt="Deutsche Bank, China" width="280px" height="65px" align="absmiddle"></a>
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         <div align="center"><a href="https://china.db.com/index_e.html" target="_blank"><img src="https://static.igem.org/mediawiki/2013/a/a7/2013ustc-china_Db-logo.png" alt="Deutsche Bank, China" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">School of Life Sciences, USTC</p>
         <p align="center">School of Life Sciences, USTC</p>
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         <div align="center"><a href="http://en.biox.ustc.edu.cn" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/27/USTC-Software-images-life-logo.png" alt="School of Life Sciences, USTC" width="280px" height="65px" align="absmiddle"></a>
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         <div align="center"><a href="http://en.biox.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/9/98/2013ustc-china_Life-logo.png" alt="School of Life Sciences, USTC" height="65px" align="absmiddle" width="280px"></a>
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       <p align="center">USTC<span style="color:#fcc644">IF</span></p>
       <p align="center">USTC<span style="color:#fcc644">IF</span></p>
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         <div align="center"><a href="http://www.ustcif.org" target="_blank"><img src="https://static.igem.org/mediawiki/2012/d/d9/USTC-Software-images-ustcif-logo.png" width="280px" height="65px" align="absmiddle"></a>
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         <div align="center"><a href="http://www.ustcif.org/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/3/3c/2013ustc-china_Ustcif-logo.png" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">School of the Gifted Young, USTC</p>
         <p align="center">School of the Gifted Young, USTC</p>
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         <div align="center"><a href="http://en.scgy.ustc.edu.cn" target="_blank"><img src="https://static.igem.org/mediawiki/2012/f/f1/Gifted_young.jpg" alt="School of the Gifted Young, USTC" width="280px" height="65px" align="absmiddle"></a>
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         <div align="center"><a href="http://en.scgy.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/c/c1/2013ustc-china_Gifted_young.jpg" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">School of physics, USTC</p>
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         <div align="center"><a href="http://en.physics.ustc.edu.cn" target="_blank"><img src="https://static.igem.org/mediawiki/2012/f/f5/USTC-Software-images-physics-logo.png" width="280px" height="65px" align="absmiddle"></a>
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         <div align="center"><a href="http://en.physics.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/6/68/2013ustc-china_Physics-logo.png" height="65px" align="absmiddle" width="280px"></a>
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         <p align="center">Teaching Affairs Office of USTC</p>
         <p align="center">Teaching Affairs Office of USTC</p>
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         <div align="center"><a href="http://www.teach.ustc.edu.cn" target="_blank"><img src="https://static.igem.org/mediawiki/2012/2/2d/USTC-Software-images-teaching-logo.png" alt="Teaching Affairs Office Of USTC" width="280px" height="65px" style="background-color:white" align="absmiddle"></a>
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         <div align="center"><a href="http://www.teach.ustc.edu.cn/" target="_blank"><img src="https://static.igem.org/mediawiki/2013/f/f9/2013ustc-china_Teaching-logo.png" alt="Teaching Affairs Office Of USTC" style="background-color:white" height="65px" align="absmiddle" width="280px"></a>
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Latest revision as of 03:28, 29 October 2013

USTC_China

In Situ Transdermal Vaccine

2013, We Redefined Vaccine
A Revolutionary Transdermal Vaccine
Safe, Reliable, Needle-free and Available for Everyone

Project

Motivation

We hope to develop a fresh-expression transdermal vaccination.
We hope everyone in this world could equally enjoy the benefit of medical progress.
more

Design of Project

With the support of TD-1, a special polypeptide which can greatly facilitate macromolecule transdermal delivery across intact skin, we constructed three different fuctional engineered Bacillus subtilis; besides, we designed a reporter system, including a kill switch, in the fouth type B.subtilis to improve usability and ensure biosafety.
ANTIGEN
As the core of our in-situ system,
TD1-antigen expresser is designed
and will make up great percentage of
in the total bacteria in our "band-aid".
more
LTB
As one kind of our adjuvant expresser,
TD1-LTB is used to enhance the antigenicity.
For LTB has so many advantages, it's a widely used adjuvant for most of exsiting injected vaccine.
more
TNFα
TNFα is a chemotactic factor recruiting Langerhans Cells, the antigen presenting cells beneath the skin barrier. The TNFα expresser is responsible for collecting LC and triggering more intense immune responses.
more
REPORTER
Reporter notifies users the status of vaccine patch and when to stick. Furthermore, it contains killing switch as a biosafety guard.
more

Experimental Measurement

Construction of Vectors
We took E.coli and the pET vector as the positive control and realized our ideas in the
B.subtilis
expression system based on pHT43 shuttle vector.
more
Tests on fusion protein
To achieve fresh expression ultimately, we used purified protein to obtain accurate data and verify our design.
more
Immunogenicity experiments
The most prominent character of 2013USTC iGEM team is that we have not only achieved a series of molecule experiments but also conducted mice experiments, including in vitro transdermal experiments and mice experiments
more

Retrieved from "http://2013.igem.org/Team:USTC_CHINA"