Team:British Columbia/Notebook

From 2013.igem.org

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{{:Team:British_Columbia/Templates/MainHeader}}
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!align="center"|[[Team:British_Columbia|Home]]
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!align="center"|[[Team:British_Columbia/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=British_Columbia Official Team Profile]
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!align="center"|[[Team:British_Columbia/Project|Project]]
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!align="center"|[[Team:British_Columbia/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:British_Columbia/Modeling|Modeling]]
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!align="center"|[[Team:British_Columbia/Notebook|Notebook]]
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!align="center"|[[Team:British_Columbia/Safety|Safety]]
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!align="center"|[[Team:British_Columbia/Attributions|Attributions]]
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==Team CRISPR==
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=Notebook=
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List of BioBricks that are/will be on the way: (CAM PLEASE CONFIRM)
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Cas9:
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<br>
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1) Cas9 - Joe and Anna
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2) pTet -
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3) const. promoter + Cas9 - Joe
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4) promoter + Cas9 + Leader + R + spacer + R (on Kan)
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5) L + R + spacer + R
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Target:
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1) tracerRNA
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2) repeat + spacer + repeat - Vanins*2 + underlings
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3) leader - Ray, Fisal, Dan
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Decoy:
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<html>
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1) PAM + spacer (on Amp)
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.icon { width: 180px; margin 5px;}
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==Team Caffeine==
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The CRISPR, Flavouring, and Caffeine teams spent numerous hours in the lab to produce the results presented throughout the project. We tracked our progress through daily updates in our lab notebooks, which enabled the rest of the team to follow the work being done. This notebook is a compilation of the daily activities, procedure, and updates of these groups. <b>Follow the progress of each group in their notebook or look up the specific protocols they used and learn how to do it yourself by clicking the buttons below!</b>
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===June 20===
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Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.
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We're making a start - what are the other groups up to?
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<br>
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--grace.yi
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    <a href="https://2013.igem.org/Team:British_Columbia/Notebook/CRISPR"><img class="icon" src="https://static.igem.org/mediawiki/2013/a/a9/Crispr2-01.png"
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        onmouseover="this.src='https://static.igem.org/mediawiki/2013/f/fa/Crispr_hover-01.png'"
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    <a href="https://2013.igem.org/Team:British_Columbia/Notebook/Flavours">
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        <img class="icon" src="https://static.igem.org/mediawiki/2013/9/96/UBCFlavourButton.png"
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    <a href="https://2013.igem.org/Team:British_Columbia/Notebook/Caffeine">
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        <img class="icon" src="https://static.igem.org/mediawiki/2013/d/d3/Caffeine2-01.png"
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  <a href="https://2013.igem.org/Team:British_Columbia/Notebook/Protocols">
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        <img class="icon" src="https://static.igem.org/mediawiki/2013/3/3b/UBCProtocols2.png"
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        onmouseover="this.src='https://static.igem.org/mediawiki/2013/b/b5/UBCProtocols.png'"
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        onmouseout="this.src='https://static.igem.org/mediawiki/2013/3/3b/UBCProtocols2.png'"/></a>
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</center>
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Latest revision as of 03:48, 29 October 2013

iGEM Home

Notebook



The CRISPR, Flavouring, and Caffeine teams spent numerous hours in the lab to produce the results presented throughout the project. We tracked our progress through daily updates in our lab notebooks, which enabled the rest of the team to follow the work being done. This notebook is a compilation of the daily activities, procedure, and updates of these groups. Follow the progress of each group in their notebook or look up the specific protocols they used and learn how to do it yourself by clicking the buttons below!





Retrieved from "http://2013.igem.org/Team:British_Columbia/Notebook"