Team:British Columbia/Notebook

From 2013.igem.org

(Difference between revisions)
(→Team Caffeine)
 
(66 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:British_Columbia/Templates/MainHeader}}
{{:Team:British_Columbia/Templates/MainHeader}}
-
==Team CRISPR==
+
=Notebook=
-
===July 3===
+
-
List of BioBricks that are/will be on the way:
+
<br>
-
Cas9:
+
<br>
-
*Cas9 - Joe and Anna
+
-
*pTet -
+
-
*const. promoter + Cas9 - Joe
+
-
*promoter + Cas9 + Leader + R + spacer + R (on Kan)
+
-
*L + R + spacer + R
+
-
Target:
+
<html>
-
*tracerRNA
+
<style>
-
*repeat + spacer + repeat - Vanins*2 + underlings
+
.icon { width: 180px; margin 5px;}
-
*leader - Ray, Fisal, Dan
+
</style>
-
Decoy:
 
-
*PAM + spacer (on Amp)
 
-
==Team Cinnamaldehyde==
+
The CRISPR, Flavouring, and Caffeine teams spent numerous hours in the lab to produce the results presented throughout the project. We tracked our progress through daily updates in our lab notebooks, which enabled the rest of the team to follow the work being done. This notebook is a compilation of the daily activities, procedure, and updates of these groups. <b>Follow the progress of each group in their notebook or look up the specific protocols they used and learn how to do it yourself by clicking the buttons below!</b>
-
4-coumarate-CoA ligase:
+
-
*PCR worked
+
-
==Team Caffeine==
+
<br>
-
===June 20, 2013===
+
<br>
-
Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.
+
-
We're making a start - what are the other groups up to?
 
-
- Grace Yi
+
<div>
 +
<center>
 +
    <a href="https://2013.igem.org/Team:British_Columbia/Notebook/CRISPR"><img class="icon" src="https://static.igem.org/mediawiki/2013/a/a9/Crispr2-01.png"
 +
        onmouseover="this.src='https://static.igem.org/mediawiki/2013/f/fa/Crispr_hover-01.png'"
 +
        onmouseout="this.src='https://static.igem.org/mediawiki/2013/a/a9/Crispr2-01.png'"/></a>
 +
    <a href="https://2013.igem.org/Team:British_Columbia/Notebook/Flavours">
 +
        <img class="icon" src="https://static.igem.org/mediawiki/2013/9/96/UBCFlavourButton.png"
 +
        onmouseover="this.src='https://static.igem.org/mediawiki/2013/e/e1/UBCFlavoursButton2.png'"
 +
        onmouseout="this.src='https://static.igem.org/mediawiki/2013/9/96/UBCFlavourButton.png'"/></a>
 +
    <a href="https://2013.igem.org/Team:British_Columbia/Notebook/Caffeine">
 +
        <img class="icon" src="https://static.igem.org/mediawiki/2013/d/d3/Caffeine2-01.png"
 +
        onmouseover="this.src='https://static.igem.org/mediawiki/2013/9/9c/Caffeine_hover-01.png'"
 +
        onmouseout="this.src='https://static.igem.org/mediawiki/2013/d/d3/Caffeine2-01.png'"/></a>
 +
  <a href="https://2013.igem.org/Team:British_Columbia/Notebook/Protocols">
 +
        <img class="icon" src="https://static.igem.org/mediawiki/2013/3/3b/UBCProtocols2.png"
 +
        onmouseover="this.src='https://static.igem.org/mediawiki/2013/b/b5/UBCProtocols.png'"
 +
        onmouseout="this.src='https://static.igem.org/mediawiki/2013/3/3b/UBCProtocols2.png'"/></a>
 +
</center>
 +
</div>
-
===June 27, 2013===
 
-
TU Munich Parts that we received were transformed into these competent cells, and transformation efficiency was very low, giving us two colonies per plate. The three parts (17H, 17J, 17L) were miniprepped, with DNA concentration of 154.3ng/ul, 323.8ng/ul and 117.3ng/ul respectively.
 
-
- Liz Geum
+
</html>
-
===July 4, 2013===
+
<br>
-
We ran an initial PCR on the miniprepped plasmids with biobrick primers (VF2 and VR) to confirm the presence of the parts. Gel confirmation of the PCR products showed a band near 1kb, our expected gene size, for one of the genes only. The other samples did not show any bands. Because the transformation efficiency was very low, we decided to troubleshoot transformation with new competent cells we received from Ray, our grad advisor.
+
-
We also designed with Ray and ordered the"stitching" primers that would allow us to assemble our biobricks easily without having to standard-assemble. In the design, we included a consensus sequence to allow for polycistronic transcription.
+
-
- Liz Geum
+
<br>
-
===July 9, 2013===
+
 
-
Transformation with new competent cells was highly efficient.
+
 
 +
<br>

Latest revision as of 03:48, 29 October 2013

iGEM Home

Notebook



The CRISPR, Flavouring, and Caffeine teams spent numerous hours in the lab to produce the results presented throughout the project. We tracked our progress through daily updates in our lab notebooks, which enabled the rest of the team to follow the work being done. This notebook is a compilation of the daily activities, procedure, and updates of these groups. Follow the progress of each group in their notebook or look up the specific protocols they used and learn how to do it yourself by clicking the buttons below!





Retrieved from "http://2013.igem.org/Team:British_Columbia/Notebook"