Team:UC Davis

From 2013.igem.org

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<h1>Welcome</h1>
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<p><img src="https://static.igem.org/mediawiki/2013/2/2a/IGEM_2013_Group_Photo.png" width="200" height="300" align="left"></a>Welcome to the 2013 UC Davis iGEM Wiki!<br></br>We have a created a novel class of transcription factors known as RiboTALs. We sought to address the constraints placed on circuit design by the limited number of well characterized promoters at our disposal, and their respective transcription factors. TetR, LacI, AraC, LuxR...do these sound familiar? What if we had transcriptional regulators that could be used in any chassis, any strain? What if we could directly engineer repressors for target sequences, instead of having to assemble parts so as to place them under control of a promoter? Furthermore, what if we could control this repression system with a molecule of choice? We'd have the ability to host multiple, orthogonal systems within the same chassis. The need to 'bioprospect' metabolites would diminish. A large part of synthetic biology is, ultimately, designing constructs that generate a response to an input stimulus. A construct that is entirely flexible both at its inputs and outputs is the ideal tool to facilitate the engineering of synthetic biology devices. Finally, what if we could increase the degrees of freedom that we as researchers have in the control of gene expression pathways? If we decoupled transcription and translation of a repressor device, maintaining fine-tuned control of both processes, and characterized the behavior of all the parts involved, the dynamic range achievable would be stupendous.</br>
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<br>Our device generates Transcriptional Activator-Like (TAL) effectors which can be engineered to bind to and repress any sequence of interest. The production of these repressor proteins is controlled by riboswitches, and thus inherit the potential of riboswitches to respond to any inducer molecule due to their engineerable and modular aptamer binding domains. <br></br> We also created a Biobrick Characterization Data Depot for more thorough characterization of the Registry's Biobricks. We hope that future iGEM teams may use this database to better understand a Biobrick's functionality.</p>
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Tweets by @UCDavisiGEM</a>
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                     <a href="https://2013.igem.org/Team:UC_Davis/Project"><div>
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                     <div><a href="https://2013.igem.org/Team:UC_Davis/Project_Overview">
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                     <a href="https://2013.igem.org/Team:UC_Davis/Project"><h3>Project Background</h3></a>
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                     <a href="https://2013.igem.org/Team:UC_Davis/Project_Overview"><h3>Project Overview</h3></a>
                     <p>Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for    inducible repression.  
                     <p>Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for    inducible repression.  
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               <a href="https://2013.igem.org/Team:UC_Davis/Data"><h3>Results</h3></a>
               <a href="https://2013.igem.org/Team:UC_Davis/Data"><h3>Results</h3></a>
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                     <p>Check out the results of our experiments.  
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                     <p>Check out the cool results of our experiments with RiboTALs. <br />
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                            <a href="https://2013.igem.org/Team:UC_Davis/AndersonPromoters"><img src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png" class="checkmark"></a><p>We also made the Anderson promoter family controllable through induction with RiboTALs.</p>
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                     <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><img src="https://static.igem.org/mediawiki/igem.org/0/00/UCD_Outreach_2013.png" class="blur"></a>
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                     <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><h3>Human Practices</h3></a>
                     <a href="https://2013.igem.org/Team:UC_Davis/HumanPracticesOverview"><h3>Human Practices</h3></a>
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                     <p>Take a look at how we designed a new database for better raw data characterization of Biobricks.  
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                     <p>Take a look at how we promote sharing in iGEM through The Depot, an open BioBrick characterization database.<br />
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                        <a href="http://dilbert.cs.ucdavis.edu/Depot" class="bold">Visit the Depot!</a>
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Welcome to the 2013 UC Davis iGEM Wiki!<br></br>We have created a novel class of transcription factors known as RiboTALs. We sought to address the constraints placed on circuit design by the limited number of well characterized promoters at our disposal, and their respective transcription factors. Our device is a hybrid system composed of two parts: Transcriptional Activator-Like (TAL) effectors which can be engineered to bind to and repress any sequence of interest and riboswitches that can respond to any inducer molecule due to their engineerable and modular aptamer binding domains. <br></br> We also designed and implemented a Biobrick characterization database called The Depot to promote sharing and openness in iGEM.</p>
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Tweets by @UCDavisiGEM</a>
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Latest revision as of 22:43, 5 December 2013

Our Sponsors

Project Overview

Learn about how we combine riboswitches and TALs into robust orthogonal mechanisms for inducible repression.

Results

Check out the cool results of our experiments with RiboTALs.

We also made the Anderson promoter family controllable through induction with RiboTALs.

Human Practices

Take a look at how we promote sharing in iGEM through The Depot, an open BioBrick characterization database.
Visit the Depot!

Judging Criteria

Here's the criteria that we met for this year's team.

Welcome

Welcome to the 2013 UC Davis iGEM Wiki!

We have created a novel class of transcription factors known as RiboTALs. We sought to address the constraints placed on circuit design by the limited number of well characterized promoters at our disposal, and their respective transcription factors. Our device is a hybrid system composed of two parts: Transcriptional Activator-Like (TAL) effectors which can be engineered to bind to and repress any sequence of interest and riboswitches that can respond to any inducer molecule due to their engineerable and modular aptamer binding domains.

We also designed and implemented a Biobrick characterization database called The Depot to promote sharing and openness in iGEM.

Retrieved from "http://2013.igem.org/Team:UC_Davis"