Team:Freiburg

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ABSTRACT
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  <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/1#abstract"> Abstract </a> </div>
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  Our Team developed a universal toolkit,  termed uniCAS, that enables customizable gene   
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    regulation in mammalian cells.  Therefore, we engineered the recently understood and highly promising 
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    CRISPR/Cas9 system. <br>
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      The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences.
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      Our toolkit comprises not only a standardized dCas9 protein,  but also different effector domains for
 +
      efficient gene activation or repression.
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</div>
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  <img id="klammer_gross_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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PROJECT
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    <div id="text_gross" align="left"><a href="https://2013.igem.org/Team:Freiburg/Project/1"> Our Project </a></div>
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  <div>
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<ul style="margin-left:40px;">
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<li style="margin-top:-30px; padding-bottom:12px;"><a href="https://2013.igem.org/Team:Freiburg/Project/effector#activation">
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<span> Activation </span>
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<img  src="https://static.igem.org/mediawiki/2013/0/03/Freiburg_2013_main2_Activation.png" style="height:65px;">
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</li>
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</a>
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<li style="margin-left: -40px; padding-bottom:12px;"><a href="https://2013.igem.org/Team:Freiburg/Project/effector#repression">
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<span> Repression </span>
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<img  src="https://static.igem.org/mediawiki/2013/e/e7/Repression_freiburg_13.png" style="height:55px;">
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</li>
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</a>
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<li style="margin-bottom:-30px;"><a href="https://2013.igem.org/Team:Freiburg/Project/effector#epigenetics">
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<span> Epigenetics </span>
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<img src="https://static.igem.org/mediawiki/2013/3/31/Freiburg_2013_main2_Epigenetic.png">
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</li>
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</a>
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<ul style="margin-left:160px">
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<li style="margin-top: -30px; padding-bottom:10px;"><a href="https://2013.igem.org/Team:Freiburg/Project/crrna">
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  <img src="https://static.igem.org/mediawiki/2013/b/bb/Freiburg_2013_main2_Multiple_Targeting.png">
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<span> Multiple targeting </span>
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</li>
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</a>
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<li style="margin-left: 40px; padding-bottom:8px;"><a href="https://2013.igem.org/Team:Freiburg/Project/method">
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  <img src="https://static.igem.org/mediawiki/2013/e/ef/UniBAss_freiburg_13.png" style="height:68px;">
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<span> uniBAss</span>
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</li>
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</a>
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<li style="margin-bottom:-30px;"><a href="https://2013.igem.org/Team:Freiburg/Project/induction#light">
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  <img src="https://static.igem.org/mediawiki/2013/9/9a/Freiburg_2013_main2_Licht.png" style="height:57px;">
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<span> Light induction </span>
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</li>
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</a>
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</ul>
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HIGHLIGHTS
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      <div id="text_gross" align="left"><a style="position:absolute; top:678px; left:60px; z-index:2" href="https://2013.igem.org/Team:Freiburg/Highlights"> Highlights </div> </a>
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  <p style="position:absolute; top:720px; left:60px" > All about our <br> project in one <br> slide show! </p>
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<img src="https://static.igem.org/mediawiki/2013/0/0e/Freiburg_2013_main2_Highlights5.png" height="190" style="position:absolute; top:614px; left:105px; z-index:1">  
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TOOLKIT
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  <div id="text-box4">
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      <div id="text_gross" align="left"> <a href="https://2013.igem.org/Team:Freiburg/Project/toolkit">Manual </a></div>
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    Use our Toolkit <br> to regulate your <br> genes!
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    <img src="https://static.igem.org/mediawiki/2013/5/5d/Freiburg_2013_main2_Toolkitnew.png" height="90" style="position:absolute; top:715px; left:464px">
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HUMAN PRACTICE
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      <div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:660px; z-index:2" href="https://2013.igem.org/Team:Freiburg/HumanPractice"> Human <br><br> Practice </a> </div>
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  <img src="https://static.igem.org/mediawiki/2013/e/ee/Freibrug_startseite_Humanpractice.png" height="150" style="position:absolute; top:650px; left:655px; z-index:1">
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TEAM
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  <div id="text-box6">
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<div id="text_gross" align="left"> <a style="position:absolute; top:678px; left:910px; z-index:2" href="https://2013.igem.org/Team:Freiburg/News"> News  </a></div>
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<p style="padding-top:10px;">
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Have a look at our achievements in Lyon & Boston!
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</p>
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<img src="https://static.igem.org/mediawiki/2013/5/56/Best-foundational-advance-2013-freiburg.png" height="130" style="position:absolute; top:600px; left:980px; z-index:1">
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  </div>
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  <img id="klammer_klein_rechts" src="https://static.igem.org/mediawiki/2013/8/82/Freiburg_2013_main2_Rahmen_gross_rechts.png">
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Freiburg_logo.png|200px|right|frame]]
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|-
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Freiburg_team.png|right|frame|Your team picture]]
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|-
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|align="center"|[[Team:Freiburg | Team Freiburg]]
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|}
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Freiburg|Home]]
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</html>
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!align="center"|[[Team:Freiburg/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Freiburg Official Team Profile]
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!align="center"|[[Team:Freiburg/Project|Project]]
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!align="center"|[[Team:Freiburg/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Freiburg/Modeling|Modeling]]
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!align="center"|[[Team:Freiburg/Notebook|Notebook]]
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!align="center"|[[Team:Freiburg/Safety|Safety]]
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!align="center"|[[Team:Freiburg/Attributions|Attributions]]
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Latest revision as of 23:52, 9 April 2014


Abstract
Our Team developed a universal toolkit, termed uniCAS, that enables customizable gene regulation in mammalian cells. Therefore, we engineered the recently understood and highly promising CRISPR/Cas9 system.
The regulation is based on the RNA-guided dCas9 protein, which allows specific targeting of DNA sequences. Our toolkit comprises not only a standardized dCas9 protein, but also different effector domains for efficient gene activation or repression.
Our Project
Highlights

All about our
project in one
slide show!

Manual
Use our Toolkit
to regulate your
genes!
Human

Practice
News

Have a look at our achievements in Lyon & Boston!

Retrieved from "http://2013.igem.org/Team:Freiburg"