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Team:Evry/Notebook/w4
From 2013.igem.org
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<h2> Tuesday, July 9th </h2> | <h2> Tuesday, July 9th </h2> | ||
| - | |||
| - | <img src="https://static.igem.org/mediawiki/2013/e/e1/WP_20130711_004.jpg" alt="Petri box" width=200/> | + | <table id='team' cellspacing='10' align='center'> |
| - | <img src="https://static.igem.org/mediawiki/2013/b/b7/WP_20130711_006.jpg" alt="Petri box" width=192/></p>< | + | <tr> |
| + | <td> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/e/e1/WP_20130711_004.jpg" alt="Petri box" width=200/> | ||
| + | <img src="https://static.igem.org/mediawiki/2013/b/b7/WP_20130711_006.jpg" alt="Petri box" width=192/> | ||
| + | <p> | ||
| + | Both strain are not contaminated. | ||
| + | </p> | ||
| + | </td> | ||
| - | < | + | <td> |
| - | <img src="https://static.igem.org/mediawiki/2013/f/f1/WP_20130711_002.jpg" alt="Petri box" width=170/> | + | <img src="https://static.igem.org/mediawiki/2013/f/f1/WP_20130711_002.jpg" alt="Petri box" width=170/> |
| - | <img src="https://static.igem.org/mediawiki/2013/c/cf/WP_20130711_003.jpg" alt="Petri box" width=151/></p> | + | <img src="https://static.igem.org/mediawiki/2013/c/cf/WP_20130711_003.jpg" alt="Petri box" width=151/> |
| + | <p> | ||
| + | BL21 are highly competent, BG1655 are little competent. | ||
| + | </p> | ||
| + | </td> | ||
| + | </tr> | ||
| + | </table> | ||
<h2> Wednesday, July 10th </h2> | <h2> Wednesday, July 10th </h2> | ||
Revision as of 16:13, 26 July 2013
4: 8th July - 14th July
Monday, July 8th
We prepared the BL21 and BG1655 strains to be competent.
Tuesday, July 9th
Both strain are not contaminated. |
BL21 are highly competent, BG1655 are little competent. |
Wednesday, July 10th
PCR procedure optimization
But : Déterminer le nombre de matrice d'ADN optimal pour amplifier un gène par PCR
Données :
Pour les PCR nous avons choisi d'utiliser --- ng de primer (V = --- µl). Connaissant la longueur de nos primer, nous avons pu déterminer le nombre de primer que nous utilisons lors de la réaction.
Grâce à la formule suivante nous avons déterminer le nombre de matrice à prélever en fonction de la concentration de l'échantillon.
AJOUTER LA FORMULE
Analyse des résultats sur gel d'agarose :
AJOUTER LA PHOTO LEGENDEE
