Team:Leeds/Results

From 2013.igem.org

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We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth.
We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth.
===Control Bacterial Growth Curve===
===Control Bacterial Growth Curve===
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[[File:Leeds_Growthcurvecontrol.png|Right||300px|Control gorwth curve|frameless]]This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device.
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[[File:Leeds_Growthcurvecontrol.png|right|450px|Control growth-curve for Competent Cells|frameless]]This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device.
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==Agarose Gels For Digestions==
==Agarose Gels For Digestions==
For every Digestion we do we will run a gel to ensure the digestion was successful, and use gel extraction kits to obtain purified DNA.
For every Digestion we do we will run a gel to ensure the digestion was successful, and use gel extraction kits to obtain purified DNA.
===Digestion of plasmid containing Green Flourescent Protein===
===Digestion of plasmid containing Green Flourescent Protein===
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The part [http://parts.igem.org/Part:BBa_K081012:Design K081012] was digested with Ecor1 and a gel run to make sure the fragment(s)are of the correct length.
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The part [http://parts.igem.org/Part:BBa_K081012:Design K081012 BBa_K081012] was digested with EcoR1 and a gel run to make sure the fragment(s)are of the correct length.
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[[File:Leeds_Agarosegelforgfp.png|left|300px|Agarose gel of GFP Digestion with Ecor1|frameless]] This gel shows the bands obtained by Ecor1 digestion of BBa-K081012. There are 100bp ladders and 1kbp ladders. This gel was used to work out the size of the fragments obtained.
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[[File:Leeds_Agarosegelforgfp.png|left|300px|Agarose gel of GFP Digestion with EcoR1|frameless]] This gel shows the bands obtained by Ecor1 digestion of BBa-K081012. There are 100bp ladders and 1kbp ladders. This gel was used to work out the size of the fragments obtained.
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===DNA Ladder Calibration Graph===
===DNA Ladder Calibration Graph===
[[File:Leeds_DNAladdergraph.png|right|300px|DNA Ladder Calibration Graph for GFP Digestion|frameless]]From this graph we can work out that one fragment is around 3000bp long the other appears to be longer than the plasmid so we can assume this is undigested plasmid. The plasmid is 2858bp long. Through digestion it has been linearised by a single Ecor1 digest. The fragment corresponds to what we expect so our digest was succesful. Although not all of the plasmid was digested.}}
[[File:Leeds_DNAladdergraph.png|right|300px|DNA Ladder Calibration Graph for GFP Digestion|frameless]]From this graph we can work out that one fragment is around 3000bp long the other appears to be longer than the plasmid so we can assume this is undigested plasmid. The plasmid is 2858bp long. Through digestion it has been linearised by a single Ecor1 digest. The fragment corresponds to what we expect so our digest was succesful. Although not all of the plasmid was digested.}}

Revision as of 14:25, 2 August 2013

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Back to iGEM Main Page
Experimental Results
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 Here are Our Results from the lab so far


Bacterial growth curves

We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth.

Control Bacterial Growth Curve

Control growth-curve for Competent Cells
This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device.


Agarose Gels For Digestions

For every Digestion we do we will run a gel to ensure the digestion was successful, and use gel extraction kits to obtain purified DNA.

Digestion of plasmid containing Green Flourescent Protein

The part [http://parts.igem.org/Part:BBa_K081012:Design K081012 BBa_K081012] was digested with EcoR1 and a gel run to make sure the fragment(s)are of the correct length.

Agarose gel of GFP Digestion with EcoR1
This gel shows the bands obtained by Ecor1 digestion of BBa-K081012. There are 100bp ladders and 1kbp ladders. This gel was used to work out the size of the fragments obtained.


DNA Ladder Calibration Graph

DNA Ladder Calibration Graph for GFP Digestion
From this graph we can work out that one fragment is around 3000bp long the other appears to be longer than the plasmid so we can assume this is undigested plasmid. The plasmid is 2858bp long. Through digestion it has been linearised by a single Ecor1 digest. The fragment corresponds to what we expect so our digest was succesful. Although not all of the plasmid was digested.
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