Team:Paris Saclay/Notebook/August/8
From 2013.igem.org
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='''Notebook : August 8'''= | ='''Notebook : August 8'''= | ||
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=='''Lab work'''== | =='''Lab work'''== | ||
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+ | ==='''Aerobic/Anaerobic regulation system'''=== | ||
+ | ===='''Preparation of the PSB3K3 backbone plasmid'''==== | ||
- | + | =====Electrophoresis of BBa_J004450 digested by EcoRI/Pst1===== | |
+ | Damir, Nadia | ||
- | + | '''Experiment''' | |
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+ | {| | ||
+ | | style="width:350px;border:1px solid black;" | IMAGE | ||
+ | | style="width:350px;border:1px solid black;" | | ||
+ | Well 1 : 6µL DNA Ladder | ||
+ | Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 | ||
+ | Gel : 0.8% | ||
+ | |} | ||
+ | '''Analysis''' | ||
+ | Expected sizes : | ||
- | + | PSB3K3 : 2750kb | |
- | + | GFP : 1069kb | |
+ | We obtained fragments of the right size. | ||
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{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} |
Revision as of 14:21, 9 August 2013
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
Electrophoresis of BBa_J004450 digested by EcoRI/Pst1
Damir, Nadia
Experiment
IMAGE |
Well 1 : 6µL DNA Ladder Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 Gel : 0.8% |
Analysis
Expected sizes :
PSB3K3 : 2750kb GFP : 1069kb
We obtained fragments of the right size.