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Team:Paris Saclay/Notebook/August/8
From 2013.igem.org
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=====1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right===== | =====1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right===== | ||
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Gel : 0.8% | Gel : 0.8% | ||
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We obtained fragments of the right size. | We obtained fragments of the right size. | ||
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Gel : 0.8% | Gel : 0.8% | ||
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Revision as of 14:29, 9 August 2013
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right
Damir, Nadia
| IMAGE |
Well 1 : 6µL DNA Ladder Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 Gel : 0.8% |
Expected sizes :
PSB3K3 : 2750kb GFP : 1069kb
We obtained fragments of the right size.
2 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to purify the plasmid
Anaïs
| IMAGE |
Well 1 : 6µL DNA Ladder Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 Gel : 0.8% |
Expected sizes :
PSB3K3 : 2750kb GFP : 1069kb
We obtained fragments of the right size.
