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Team:Paris Saclay/Notebook/August/8
From 2013.igem.org
(Difference between revisions)
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| style="width:350px;border:1px solid black;" | IMAGE | | style="width:350px;border:1px solid black;" | IMAGE | ||
| style="width:350px;border:1px solid black;" | | | style="width:350px;border:1px solid black;" | | ||
| - | + | *Well 1 : 6µL DNA Ladder | |
| - | + | *Well 2 : 5µL of BBa_J004450 digested by EcoRI/Pst1 | |
| - | + | *Gel : 0.8% | |
|} | |} | ||
Expected sizes : | Expected sizes : | ||
| - | + | *PSB3K3 : 2750kb | |
| - | + | *GFP : 1069kb | |
| - | We obtained fragments of the right size. | + | We obtained fragments of the right size. Now we can purify the PSB3K3 plasmid. |
| - | =====2 - | + | =====2 - Gel purification of the PSB3K3 plasmid from BBa_J004450 digested by EcoRI/Pst1===== |
Anaïs | Anaïs | ||
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| style="width:350px;border:1px solid black;" | IMAGE | | style="width:350px;border:1px solid black;" | IMAGE | ||
| style="width:350px;border:1px solid black;" | | | style="width:350px;border:1px solid black;" | | ||
| - | + | *Well 1 : 6µL DNA Ladder | |
| - | + | *Well 2 : 45µL of BBa_J004450 digested by EcoRI/Pst1 | |
| - | + | *Gel : 0.8% | |
|} | |} | ||
| - | + | Now we can do a gel purification of the highest band. | |
| + | |||
| + | =====3 - Electro-elution of the plasmid PSB3K3===== | ||
| + | Nadia | ||
| + | |||
| - | + | Now we can do a gel purification of the highest band. | |
| - | + | ||
| - | |||
Revision as of 14:40, 9 August 2013
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right
Damir, Nadia
| IMAGE |
|
Expected sizes :
- PSB3K3 : 2750kb
- GFP : 1069kb
We obtained fragments of the right size. Now we can purify the PSB3K3 plasmid.
2 - Gel purification of the PSB3K3 plasmid from BBa_J004450 digested by EcoRI/Pst1
Anaïs
| IMAGE |
|
Now we can do a gel purification of the highest band.
3 - Electro-elution of the plasmid PSB3K3
Nadia
Now we can do a gel purification of the highest band.
