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Team:Paris Saclay/Notebook/August/8
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Now we can do a gel purification of the highest band which contains the PSB3K3 plasmid. | Now we can do a gel purification of the highest band which contains the PSB3K3 plasmid. | ||
| - | ''2.2 - | + | ''2.2 - Electroelution of the highest band to extract the PSB3K3 plasmid from the gel'' |
Nadia | Nadia | ||
| + | Protocol : [[Team:Paris_Saclay/Protocols/Electroelution|Electroelution]] | ||
| + | |||
| + | We let the plasmid precipitate during the night. | ||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 14:56, 9 August 2013
Contents |
Notebook : August 8
Lab work
Aerobic/Anaerobic regulation system
Preparation of the PSB3K3 backbone plasmid
1 - Electrophoresis of BBa_J004450 digested by EcoRI/Pst1 to check if the digestion was right
Damir, Nadia
| IMAGE |
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Expected sizes :
- PSB3K3 : 2750kb
- GFP : 1069kb
We obtained fragments of the right size. Now we can purify the PSB3K3 plasmid.
2 - Gel purification of the PSB3K3 plasmid from BBa_J004450 digested by EcoRI/Pst1
2.1 - Migration of the remaining 45µL of BBa_J004450 digested by EcoRI/Pst1
Anaïs
| IMAGE |
|
Now we can do a gel purification of the highest band which contains the PSB3K3 plasmid.
2.2 - Electroelution of the highest band to extract the PSB3K3 plasmid from the gel
Nadia
Protocol : Electroelution
We let the plasmid precipitate during the night.
