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Team:Leeds/Results
From 2013.igem.org
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We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth. | We are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth. | ||
===Control Bacterial Growth Curve=== | ===Control Bacterial Growth Curve=== | ||
| - | [[File:Leeds_Growthcurvecontrol.png|right|450px|Control growth-curve for Competent Cells|frameless]]This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device. | + | [[File:Leeds_Growthcurvecontrol.png|right|450px|Control growth-curve for Competent Cells|frameless]] |
| + | <br>This growth curve shows the lag phase and log phase of untransformed bacteria. The graph also shows that the bacteria do not grow in the presence of chloramphenecol. Our bacterial device will grow in the presence of chloramphenecol. We will use this graph as a comparison with our bacterial device. | ||
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==Agarose Gels For Digestions== | ==Agarose Gels For Digestions== | ||
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===Digestion of plasmid containing Green Flourescent Protein=== | ===Digestion of plasmid containing Green Flourescent Protein=== | ||
The part [http://parts.igem.org/Part:BBa_K081012:Design K081012] was digested with EcoR1 and a gel run to make sure the fragment(s)are of the correct length. | The part [http://parts.igem.org/Part:BBa_K081012:Design K081012] was digested with EcoR1 and a gel run to make sure the fragment(s)are of the correct length. | ||
| - | [[File:Leeds_Agarosegelforgfp.png|left|300px|Agarose gel of GFP Digestion with EcoR1|frameless]] This gel shows the bands obtained by | + | [[File:Leeds_Agarosegelforgfp.png|left|300px|Agarose gel of GFP Digestion with EcoR1|frameless]] This gel shows the bands obtained by EcoR1 digestion of BBa-K081012. There are 100bp ladders and 1kbp ladders. This gel was used to work out the size of the fragments obtained. |
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===DNA Ladder Calibration Graph=== | ===DNA Ladder Calibration Graph=== | ||
Revision as of 18:50, 13 August 2013
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 | Here are Our Results from the lab so far
Bacterial growth curvesWe are monitoring the growth of our bacterial devices to see if any of the genes we have inserted have an effect on bacterial growth. Control Bacterial Growth Curve
Agarose Gels For DigestionsFor every Digestion we do we will run a gel to ensure the digestion was successful, and use gel extraction kits to obtain purified DNA. Digestion of plasmid containing Green Flourescent ProteinThe part [http://parts.igem.org/Part:BBa_K081012:Design K081012] was digested with EcoR1 and a gel run to make sure the fragment(s)are of the correct length. This gel shows the bands obtained by EcoR1 digestion of BBa-K081012. There are 100bp ladders and 1kbp ladders. This gel was used to work out the size of the fragments obtained.
DNA Ladder Calibration Graph
Characterisation of Device 1
Growth Curve
Restriction Digests
Plate Imaging
Membrane Stress- Hydrophobic Stress
Membrane Stress- Changes in pH
Membrane Stress- Atomic Force Microscopy
Characterisation of Device 2
Growth Curve
Restriction Digests
Plate Imaging
Binding assay-washing beads
Binding assay-Confocal Microscopy
Characterisation of Device 3
Growth Curve
Restriction Digests
Plate Imaging
Binding Assay-Beads cause fluorescence? | ||||||
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