Timer Plus Sumo

From 2013.igem.org

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<p>Figure 1: Circuit of the timer inluding sumo cleaving</p></div>
<p>Figure 1: Circuit of the timer inluding sumo cleaving</p></div>
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<h2 align="center">Differential Equations</h2>
<h2 align="center">Differential Equations</h2>
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The above circuit can be represented by the following di�erential equations. We assume a binary
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behavior of the T7 promoter. In the presence of IPTG, the T7 promoter will be active. So, we make
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the assumption that the T7 is binary variable with two possible states: either active 1 or inactive 0,
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this is the variable s.
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Revision as of 14:15, 15 August 2013

Timer Plus Sumo


In this section the system of Figure 1 is modeled. The structure of the timer is very similar version of the timer compared to the construct of iGEM TU Delft team 2009. Here the input is changed to a T7 promoter and the output to Ulp-1. Furthermore, the Ulp-1 cleaves off the SUMO from the peptide combined with the SUMO.


Figure 1: Circuit of the timer inluding sumo cleaving











Differential Equations

The above circuit can be represented by the following di�erential equations. We assume a binary behavior of the T7 promoter. In the presence of IPTG, the T7 promoter will be active. So, we make the assumption that the T7 is binary variable with two possible states: either active 1 or inactive 0, this is the variable s.