16/08/13

From 2013.igem.org

(Difference between revisions)
(Created page with "==Running the gel from sonication series== *Samples were taken from sonicated tube every 30min intervals, a total of 6 samples. *Each was diluted 100 fold to get a concentration ...")
 
(One intermediate revision not shown)
Line 1: Line 1:
 +
<div style="font-family:arial;padding:5px;border-radius:5px;border:5px solid #FF2800;">
 +
{| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center"
 +
!align="center"|[[Team:Leicester|Home]]
 +
!align="center"|[[Team:Leicester/Team|Team]]
 +
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile]
 +
!align="center"|[[Team:Leicester/Project|Project]]
 +
!align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]]
 +
!align="center"|[[Team:Leicester/Modeling|Modeling]]
 +
!align="center"|[[Team:Leicester/Notebook|Notebook]]
 +
!align="center"|[[Team:Leicester/Safety|Safety]]
 +
!align="center"|[[Team:Leicester/Attributions|Attributions]]
 +
|}
 +
</div>
 +
 +
 +
==Running the gel from sonication series==
==Running the gel from sonication series==
*Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
*Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
*Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
*Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
*5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
*5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
-
*Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated
+
*Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated.
-
 
+
-
[[File:igem_2nd_son_series_160813.jpg]]
+

Latest revision as of 09:18, 22 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


Running the gel from sonication series

  • Samples were taken from sonicated tube every 30min intervals, a total of 6 samples.
  • Each was diluted 100 fold to get a concentration in the range of 40-50ng/ul
  • 5ul of DNA was taken from the dilution, to which 10ul of sterile water and 5ul of orange G was added
  • Samples were run against a 1kb ladder and control herring sperm DNA, which was not sonicated.