Team:Imperial College/Results

From 2013.igem.org

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<b>Glucose growth assay</b>
<b>Glucose growth assay</b>
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[[File:Glucose_graph.png|thumbnail|center|600px|Cell growth of phaABC <i>E. coli</i> at 4 concentrations of glucose. Optimum growth is at 2-4% glucose.]]
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[[File:Glucose_graph.png|thumbnail|center|600px|Cell growth of phaABC <i>E. coli</i> at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC.]]
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<b>WCM growth assay</b>
<b>WCM growth assay</b>
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{| class="wikitable" style="margin: 1em auto 1em auto;"
{| class="wikitable" style="margin: 1em auto 1em auto;"
| [[File:Very_stressed_ecoli.jpg|thumbnail|left|400px|Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated [https://2013.igem.org/Team:Imperial_College/Protocols WCM]]]
| [[File:Very_stressed_ecoli.jpg|thumbnail|left|400px|Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated [https://2013.igem.org/Team:Imperial_College/Protocols WCM]]]
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| [[File:WCM_media.png|thumbnail|right|400px|Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated ]]
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| [[File:WCM_media.png|thumbnail|right|400px|Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC]]
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|}
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<b>Ethylene glycol growth assay</b>
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[[File:EG_growth.png|thumbnail|center|600px|Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0µl, 100µl and 200µl at 30ºC.]]
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Revision as of 18:57, 22 August 2013

Results


Glucose growth assay

Cell growth of phaABC E. coli at 4 concentrations of glucose. Optimum growth is at 2-4% glucose at 37ºC.



WCM growth assay

Production of the red pigment by stress induction. MG1655 were grown with LB media and sterile filtrated WCM
Growth curve of our [http://parts.igem.org/Part:BBa_K639003 mCherry] MG1655 bacteria. MG1655 were grown with LB media and sterile filtrated WCM at 37ºC



Ethylene glycol growth assay

Cell growth of MG1655 in ethylene glycol, a byproduct of polyurethane degradation. Cells were grown in 0µl, 100µl and 200µl at 30ºC.

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